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Long non-coding RNA FER1L4 inhibits prostate cancer progression via sponging miR-92a-3p and upregulation of FBXW7
Cancer Cell International ( IF 5.3 ) Pub Date : 2020-02-28 , DOI: 10.1186/s12935-020-1143-0
Wei Huo 1 , Fei Qi 2 , Kaichen Wang 1
Affiliation  

Dysregulation of long non-coding RNAs (lncRNAs) is involved in development of prostate cancer. However, the molecular mechanisms of many lncRNAs in prostate cancer have not been studied yet. The lncRNA Fer-1-like protein 4 (FER1L4) expression was explored in prostate tumors and normal prostate tissues by RT-qPCR and bioinformatic analysis. Overexpression of FER1L4 was performed to evaluate its role in prostate cancer cell proliferation and survival. The molecular mechanism of FER1L4 was investigated by dual luciferase reporter assay, RNA pull down assay, western blotting and RT-qPCR. It was found that FER1L4 was lower in prostate cancer tissues than normal tissues. Higher expression of FER1L4 was associated with prostate cancer tissues of early stage (AJCC stage I/II). Overexpression of FER1L4 inhibited cell proliferation and promoted cell apoptosis in prostate cancer cells. Bioinformatic analysis, RT-qPCR, RNA pull down assay and dual luciferase assay showed that FER1L4 upregulated F-box/WD repeat-containing protein 7 (FBXW7) tumor suppressor via sponging miR-92a-3p. Silencing of FBXW7 reversed the cell phenotypes caused by FER1L4 overexpression in prostate cancer cells. The data demonstrated that FER1L4, a downregulated lncRNA in prostate cancer, was pivotal for cell proliferation and survival of prostate cancer. The study provided new sights into understanding of the signaling network in prostate cancer and implied that FER1L4 might be a biomarker for patients with prostate cancer.

中文翻译:

长链非编码 RNA FER1L4 通过海绵化 miR-92a-3p 和上调 FBXW7 抑制前列腺癌进展

长链非编码 RNA (lncRNA) 的失调与前列腺癌的发展有关。然而,许多 lncRNA 在前列腺癌中的分子机制尚未得到研究。通过 RT-qPCR 和生物信息学分析在前列腺肿瘤和正常前列腺组织中探索了 lncRNA Fer-1 样蛋白 4 (FER1L4) 的表达。进行 FER1L4 的过表达以评估其在前列腺癌细胞增殖和存活中的作用。通过双荧光素酶报告基因分析、RNA pull down 分析、蛋白质印迹和 RT-qPCR 研究了 FER1L4 的分子机制。发现前列腺癌组织中的FER1L4低于正常组织。FER1L4 的高表达与早期(AJCC I/II 期)前列腺癌组织相关。FER1L4的过表达抑制前列腺癌细胞的细胞增殖并促进细胞凋亡。生物信息学分析、RT-qPCR、RNA pull down 测定和双荧光素酶测定表明,FER1L4 通过海绵状 miR-92a-3p 上调 F-box/WD 重复蛋白 7 (FBXW7) 肿瘤抑制因子。FBXW7 的沉默逆转了前列腺癌细胞中由 FER1L4 过表达引起的细胞表型。数据表明,前列腺癌中下调的 lncRNA FER1L4 对前列腺癌的细胞增殖和存活至关重要。该研究为理解前列腺癌信号网络提供了新的视角,并暗示 FER1L4 可能是前列腺癌患者的生物标志物。RNA pull down 试验和双荧光素酶试验表明,FER1L4 通过海绵状 miR-92a-3p 上调 F-box/WD 重复蛋白 7 (FBXW7) 肿瘤抑制因子。FBXW7 的沉默逆转了前列腺癌细胞中由 FER1L4 过表达引起的细胞表型。数据表明,前列腺癌中下调的 lncRNA FER1L4 对前列腺癌的细胞增殖和存活至关重要。该研究为理解前列腺癌信号网络提供了新的视角,并暗示 FER1L4 可能是前列腺癌患者的生物标志物。RNA pull down 试验和双荧光素酶试验表明,FER1L4 通过海绵状 miR-92a-3p 上调 F-box/WD 重复蛋白 7 (FBXW7) 肿瘤抑制因子。FBXW7 的沉默逆转了前列腺癌细胞中由 FER1L4 过表达引起的细胞表型。数据表明,前列腺癌中下调的 lncRNA FER1L4 对前列腺癌的细胞增殖和存活至关重要。该研究为理解前列腺癌信号网络提供了新的视角,并暗示 FER1L4 可能是前列腺癌患者的生物标志物。前列腺癌中下调的 lncRNA 对前列腺癌的细胞增殖和存活至关重要。该研究为理解前列腺癌信号网络提供了新的视角,并暗示 FER1L4 可能是前列腺癌患者的生物标志物。前列腺癌中下调的 lncRNA 对前列腺癌的细胞增殖和存活至关重要。该研究为理解前列腺癌信号网络提供了新的视角,并暗示 FER1L4 可能是前列腺癌患者的生物标志物。
更新日期:2020-02-28
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