BACKGROUND Microbiota from different niches within the canine oral cavity were profiled and compared. Supragingival plaque and stimulated saliva, were collected alongside samples from the buccal and tongue dorsum mucosa, from 14 Labrador retrievers at three timepoints within a 1 month timeframe. The V3-V4 region of the 16S rRNA gene was sequenced via Illumina MiSeq. RESULTS Supragingival plaque microbiota had the highest bacterial diversity and the largest number of significant differences in individual taxa when compared to the other oral niches. Stimulated saliva exhibited the highest variability in microbial composition between dogs, yet the lowest bacterial diversity amongst all the niches. Overall, the bacteria of the buccal and tongue dorsum mucosa were most similar. CONCLUSIONS The bacterial community profiles indicated three discrete oral niches: soft tissue surfaces (buccal and tongue dorsum mucosa), hard tissue surface (supragingival plaque) and saliva. The ability to distinguish the niches by their microbiota signature offers the potential for microbial biomarkers to be identified in each unique niche for diagnostic use.

中文翻译：

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犬口腔微生物组：不同生态位的细菌种群变化。

背景 对犬口腔内不同生态位的微生物群进行了分析和比较。在 1 个月内的三个时间点，从 14 只拉布拉多猎犬的颊和舌背粘膜样本中收集龈上菌斑和刺激唾液。16S rRNA 基因的 V3-V4 区域通过 Illumina MiSeq 进行测序。结果与其他口腔生态位相比，龈上菌斑微生物群具有最高的细菌多样性和最大数量的个体分类群显着差异。受刺激的唾液在狗之间表现出最高的微生物组成变异性，但在所有生态位中细菌多样性却最低。总体而言，颊粘膜和舌背粘膜的细菌最相似。结论 细菌群落分布表明三个离散的口腔生态位：软组织表面（颊和舌背粘膜）、硬组织表面（龈上菌斑）和唾液。通过微生物群特征区分生态位的能力为在每个独特的生态位中识别微生物生物标志物以用于诊断提供了潜力。