Bactobolin is a hybrid natural product with potent cytotoxic activity. Its production from Burkholderia thailandensis was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin.

中文翻译：

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Bactobolin 的化学和生物学：与杰出的天然产物化学家 Jon Clardy 的 10 年合作。

Bactobolin 是一种混合天然产物，具有强大的细胞毒活性。2010 年，Greenberg 和 Clardy 实验室合作的一部分报道了其从 Burkholderia thailandensis 的生产。该合作引发了一系列研究，导致发现新的类似物和相关的构效关系，鉴定了 bactobolin 生物合成基因簇以及其不寻常的氨基酸构建块、抗生素的分子靶标和抗性的组装，最后是核糖体-杆菌肽复合物的 X 射线晶体结构。在此，我们回顾了导致我们目前对 bactobolin 的化学和生物学理解的合作。