Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Label-free analytical performances of a peptide-based QCM biosensor for trypsin.
Analyst ( IF 3.6 ) Pub Date : 2020-03-24 , DOI: 10.1039/d0an00308e Zong-Mu Dong 1 , Lu Cheng , Ping Zhang , Guang-Chao Zhao
Analyst ( IF 3.6 ) Pub Date : 2020-03-24 , DOI: 10.1039/d0an00308e Zong-Mu Dong 1 , Lu Cheng , Ping Zhang , Guang-Chao Zhao
Affiliation
|
A label-free biosensor was fabricated for the detection of trypsin by using a peptide-functionalized quartz crystal microbalance gold electrode. The synthetized peptide chains were immobilized tightly on the QCM electrode via a self-assembly method, which formed a thin and approximate rigid layer of peptides. The detection signal was achieved by calculating the mass changes on the QCM electrode because the peptide chains could be specifically cleaved in the carboxyl terminuses of arginine and lysine by trypsin. When gold nanoparticles were coupled to the peptide chains, the sensing signal would be amplified 10.9 times. Furthermore, the sensor interface shows a lower resonance resistance change when the peptide chain is immobilized horizontally. Independent detections in parallel on different electrodes have a wide linear range. Under the optimum conditions, the signal-amplified biosensor allowed the measurement of trypsin over the range of 0-750 ng mL-1 with a detection limit of 8.6 ng mL-1. Moreover, for screening the inhibitor of trypsin, the IC50 values were obtained to be 1.85 μg mL-1 for benzamidine hydrochloride and 20.5 ng mL-1 for the inhibitor from soybean.
中文翻译：
基于肽的 QCM 胰蛋白酶生物传感器的无标记分析性能。
使用肽功能化石英晶体微天平金电极制备了用于检测胰蛋白酶的无标记生物传感器。合成的肽链通过自组装方法紧密固定在QCM电极上，形成一层薄而近似刚性的肽层。由于胰蛋白酶可以在精氨酸和赖氨酸的羧基末端特异性切割肽链，因此通过计算QCM电极上的质量变化来获得检测信号。当金纳米颗粒与肽链偶联时，传感信号将放大10.9倍。此外，当肽链水平固定时，传感器界面表现出较低的共振电阻变化。不同电极上并行的独立检测具有较宽的线性范围。在最佳条件下，信号放大生物传感器可以测量0-750 ng mL-1范围内的胰蛋白酶，检测限为8.6 ng mL-1。此外，筛选胰蛋白酶抑制剂时，盐酸苯甲脒的IC50值为1.85 μg mL-1，大豆抑制剂的IC50值为20.5 ng mL-1。
更新日期：2020-02-27
中文翻译：
基于肽的 QCM 胰蛋白酶生物传感器的无标记分析性能。
使用肽功能化石英晶体微天平金电极制备了用于检测胰蛋白酶的无标记生物传感器。合成的肽链通过自组装方法紧密固定在QCM电极上，形成一层薄而近似刚性的肽层。由于胰蛋白酶可以在精氨酸和赖氨酸的羧基末端特异性切割肽链，因此通过计算QCM电极上的质量变化来获得检测信号。当金纳米颗粒与肽链偶联时，传感信号将放大10.9倍。此外，当肽链水平固定时，传感器界面表现出较低的共振电阻变化。不同电极上并行的独立检测具有较宽的线性范围。在最佳条件下，信号放大生物传感器可以测量0-750 ng mL-1范围内的胰蛋白酶，检测限为8.6 ng mL-1。此外，筛选胰蛋白酶抑制剂时，盐酸苯甲脒的IC50值为1.85 μg mL-1，大豆抑制剂的IC50值为20.5 ng mL-1。
京公网安备 11010802027423号