Garcinol, a polyisoprenylated benzophenone extracted from the fruit rind of Garcinia indica, is known for its antioxidant, anti-inflammatory, and anti-cancer activities. We studied the mechanism of anti-obesity activity of Garcinia indica extract standardized for 20% Garcinol (GIE). 3T3L1 cells were used to study the effects on adipogenesis in vitro, while high-fat diet-fed C57/BL6 mice were used to study the anti-obesity activity. Real time quantitative polymerase chain reaction, western blot, histochemical analysis, and ELISA were used to study the mechanism of action. GIE inhibited adipogenesis in vitro, increased uncoupling protein-1 (UCP1) and reduced the endoplasmic reticulum stress in the treated cells. Animals treated with GIE were lean despite high-fat feed and showed lower visceral fat accumulation. GIE was found to alleviate the endoplasmic reticulum stress in the adipose tissues. Our results suggest that GIE with 20% Garcinol is an attractive option for the management of obesity by regulating endoplasmic reticulum stress.

藤黄酚是从藤黄果皮中提取的聚异戊二烯基二苯甲酮，以其抗氧化，抗炎和抗癌活性而闻名。我们研究了标准化为20％藤黄酚（GIE）的藤黄提取物的抗肥胖活性机理。3T3L1细胞用于研究体外对脂肪形成的影响，高脂饮食喂养的C57 / BL6小鼠用于研究抗肥胖活性。实时定量聚合酶链反应，免疫印迹，组织化学分析和酶联免疫吸附法被用来研究其作用机理。GIE在体外抑制脂肪生成，增加解偶联蛋白-1（UCP1）并减少内质网应激的处理细胞。尽管采用高脂饲料，但接受GIE治疗的动物仍然瘦弱，并且内脏脂肪堆积降低。发现GIE减轻了脂肪组织中的内质网应激。我们的结果表明，通过调节内质网应激，含20％大蒜油的GIE是控制肥胖的诱人选择。