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The use of deuterium-labeled gamma-aminobutyric (D6-GABA) to study uptake, translocation, and metabolism of exogenous GABA in plants.
Plant Methods ( IF 4.7 ) Pub Date : 2020-02-27 , DOI: 10.1186/s13007-020-00574-9
Faraj Hijaz 1 , Nabil Killiny 1
Affiliation  

Background Exogenous application of gamma-aminobutyric acid (GABA) could relieve stress symptoms caused by abiotic stresses including anoxia, heat and cold shock, drought, and salt. However, studying translocation and metabolism of exogenous GABA is challenged by the presence of endogenous GABA. Results Herein, we used D6-GABA in order to investigate the uptake, translocation, and the metabolism of exogenous GABA in Mexican lime (Citrus aurantifolia) seedlings using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis showed that D6-GABA could be easily distinguished from the non-labeled GABA after methyl chloroformate (MCF) derivatization. The D6-GABA was detected in the cortex (phloem), inner stem (xylem), and leaves after root drench. Girdling did not affect the translocation of D6-GABA, indicating that it is mainly translocated via the xylem. In addition, D4-labled succinic acid was detected in D6-GABA-treated plants, indicating that exogenous GABA was metabolized to succinic acid. The half-life of D6-GABA in citrus was about 1.3 h, indicating a quick conversion to succinic acid. Conclusion The use of D6-GABA offers a valuable tool to study the translocation and metabolism of GABA in plants. D6-GABA and its metabolite (D4-succinic acid) can be easily distinguished from the endogenous GABA and succinic acid using GC-MS.

中文翻译:

使用氘标记的 γ-氨基丁酸 (D6-GABA) 研究植物中外源 GABA 的吸收、转运和代谢。

背景 γ-氨基丁酸(GABA)的外源应用可以缓解非生物胁迫引起的应激症状,包括缺氧、冷热休克、干旱和盐分。然而,研究外源 GABA 的易位和代谢受到内源 GABA 存在的挑战。结果在此,我们使用 D6-GABA 以使用气相色谱-质谱 (GC-MS) 研究墨西哥酸橙 (Citrus aurantifolia) 幼苗中外源 GABA 的吸收、转运和代谢。GC-MS分析表明,在氯甲酸甲酯(MCF)衍生后,D6-GABA很容易与未标记的GABA区分开来。根浸后在皮层(韧皮部)、内茎(木质部)和叶片中检测到 D6-GABA。环剥不影响 D6-GABA 的易位,表明它主要通过木质部转运。此外,在 D6-GABA 处理的植物中检测到 D4 标记的琥珀酸,表明外源 GABA 被代谢为琥珀酸。D6-GABA 在柑橘中的半衰期约为 1.3 小时,表明可快速转化为琥珀酸。结论 D6-GABA 的使用为研究 GABA 在植物中的易位和代谢提供了有价值的工具。使用 GC-MS 可以很容易地将 D6-GABA 及其代谢物(D4-琥珀酸)与内源性 GABA 和琥珀酸区分开来。结论 D6-GABA 的使用为研究 GABA 在植物中的易位和代谢提供了有价值的工具。使用 GC-MS 可以很容易地将 D6-GABA 及其代谢物(D4-琥珀酸)与内源性 GABA 和琥珀酸区分开来。结论 D6-GABA 的使用为研究 GABA 在植物中的易位和代谢提供了有价值的工具。使用 GC-MS 可以很容易地将 D6-GABA 及其代谢物(D4-琥珀酸)与内源性 GABA 和琥珀酸区分开来。
更新日期:2020-04-22
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