Background

Nanotoxicology is a major field of study that reveals hazard effects of nanomaterials on the living cells.

Methods

In the present study, Copper/Copper oxide nanoparticles (Cu/CuO NPs) were prepared by the chemical reduction method and characterized by different techniques such as: X-Ray Diffraction, Transmission and Scanning Electron Microscopy. Evaluation of the toxicity of Cu/CuO NPs was performed on 2 types of cells: human lung normal cell lines (WI-38) and human lung carcinoma cell (A549). To assess the toxicity of the prepared Cu/CuOs NPs, the two cell types were exposed to Cu/CuO NPs for 72 h. The half-maximal inhibitory concentration IC₅₀ of Cu/CuO NPs for both cell types was separately determined and used to examine the cell genotoxicity concurrently with the determination of some oxidative stress parameters: nitric oxide, glutathione reduced, hydrogen peroxide, malondialdehyde and superoxide dismutase.

Results

Cu/CuO NPs suppressed proliferation and viability of normal and carcinoma lung cells. Treatment of both cell types with their IC₅₀’s of Cu/CuO NPs resulted in DNA damage besides the generation of reactive oxygen species and consequently the generation of a state of oxidative stress.

Conclusion

Overall, it can be concluded that the IC₅₀'s of the prepared Cu/CuO NPs were cytotoxic and genotoxic to both normal and cancerous lung cells.

中文翻译：

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铜/铜氧化物纳米粒子在正常和癌细胞肺癌细胞系中的细胞毒性和遗传毒性的体外评估

背景

纳米毒理学是一个主要研究领域，揭示了纳米材料对活细胞的危害作用。

方法

在本研究中，通过化学还原方法制备了铜/铜氧化物纳米颗粒（Cu / CuO NPs），并通过诸如X射线衍射，透射和扫描电子显微镜等不同技术对其进行了表征。对两种类型的细胞进行Cu / CuO NPs毒性评估：人肺正常细胞系（WI-38）和人肺癌细胞（A549）。为了评估制备的Cu / CuOs NP的毒性，将两种细胞暴露于Cu / CuO NPs 72小时。分别确定了两种细胞类型的Cu / CuO NPs的半数最大抑制浓度IC ₅₀，并用于确定某些氧化应激参数的同时检查细胞的遗传毒性：一氧化氮，还原型谷胱甘肽，过氧化氢，丙二醛和超氧化物歧化酶。

结果

Cu / CuO NPs抑制正常和癌细胞的增殖和活力。用它们的IC ₅₀ 's Cu / CuO NPs处理两种细胞类型，除了产生活性氧种类并因此产生氧化应激状态之外，还导致DNA损伤。

结论

总体而言，可以得出结论，所制备的Cu / CuO NP的IC ₅₀对正常和癌性肺癌细胞均具有细胞毒性和遗传毒性。