Background

Selenoproteins are selenocysteine (Sec)-containing proteins that exhibit numerous physiological functions, mainly antioxidative activities. Studies have suggested that several human selenoproteins play an important role in tumor initiation and progression, including melanoma.

Methods

Using RNA-seq data set from Sequence Reads Archive (SRA) experiments published at the National Center for Biotechnology Information (NCBI), we determined the transcriptional level of the 25 selenoproteins-coding sequences found in 16 human melanoma cell lines and compared to four melanocyte controls.

Results

15 selenoprotein-coding genes were found to be expressed in melanoma and normal melanocyte cells, and their mRNA levels varied among the cell lines. Most of the human melanomas analyzed containing BRAF and NRAS mutations presented elevated levels ofSELENOI, TXNRD1, and SELENOT transcripts and decreased SELENOW and SELENON transcripts in comparison with melanocytes controls. Moreover, SELENOW, SELENON, SELENOI, TXNRD1, and SELENOT-coding transcripts were reversed when BRAF-mutated A375 cells were treated with CPI203, A771726 or Vorinostat drugs.

Conclusion

Our results indicate that melanoma cells can modify, in a different manner, the selenoprotein transcript levels, as a possible mechanism to control tumor progression. We suggest that the usage of diet and supplements containing selenium should be carefully used for patients with melanoma.

中文翻译：

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在皮肤癌衍生的细胞系中获取硒蛋白的转录状态

背景

硒蛋白是含有硒代半胱氨酸（Sec）的蛋白，具有多种生理功能，主要是抗氧化活性。研究表明，几种人类硒蛋白在肿瘤的发生和发展中起着重要作用，包括黑色素瘤。

方法

使用在国家生物技术信息中心（NCBI）上发布的序列读数档案（SRA）实验中的RNA-seq数据集，我们确定了在16种人类黑素瘤细胞系中发现的25种硒蛋白编码序列的转录水平，并与4种黑素细胞进行了比较控制。

结果

发现在黑素瘤和正常黑素细胞中表达了15种硒蛋白编码基因，并且它们的mRNA水平在各细胞系中有所不同。大多数人类黑色素瘤的分析含有BRAF和NRAS基因突变呈现的水平升高SELENOI，TXNRD1，和SELENOT成绩单和降低SELENOW和SELENON与黑素细胞对照相比的转录物。此外，当将BRAF突变的A375细胞用CPI203，A771726或Vorinostat药物处理时，SELENOW，SELENON，SELENOI，TXNRD1和SELENOT编码的转录物会逆转。

结论

我们的结果表明，黑色素瘤细胞可以以不同的方式修饰硒蛋白的转录水平，作为控制肿瘤进展的可能机制。我们建议黑色素瘤患者应谨慎使用饮食和含硒补充剂。