Abstract The harvested longan fruit are prone to senescence, leading to a decline in fruit quality like pericarp browning. There are two energy dissipative proteins involving in fruit senescence: alternative oxidase (AOX) and plant uncoupling mitochondrial protein (PUMP). The purpose of this work was to elucidate the influence of adenosine triphosphate (ATP) treatment on energy dissipation system in postharvest longan fruit during senescence. The results showed that the ATP content in postharvest longan fruit exhibited negative correlations with the expressions of DlAOX1, DlAOX2, DlPUMP3, and DlPUMP5. Moreover, compared to the control longan fruit, the treatment of exogenous ATP prevented the increases in the expressions of DlAOX1, DlAOX2, DlPUMP3, and DlPUMP5, helped maintain higher contents of ATP, ADP and higher level of energy charge, but lower respiration rate, lower cell membrane permeability, and lower pericarp browning index in postharvest longan fruit. These data indicated that the supply of exogenous ATP could reduce the induction of the expressions of DlAOX1, DlAOX2, DlPUMP3 and DlPUMP5, and consequently reduce the energy dissipation and retard the decline of energy level, which lead to the delayed senescence in postharvest longan fruit.

中文翻译：

---

ATP处理对采后龙眼果实衰老过程能量耗散系统的影响

摘要 采收后的龙眼果实容易衰老，导致果实品质下降，如果皮褐变。有两种能量耗散蛋白参与果实衰老：替代氧化酶（AOX）和植物解偶联线粒体蛋白（PUMP）。本工作的目的是阐明三磷酸腺苷（ATP）处理对采后龙眼果实衰老过程中能量耗散系统的影响。结果表明，龙眼采后ATP含量与DlAOX1、DlAOX2、DlPUMP3、DlPUMP5的表达呈负相关。此外，与对照龙眼果实相比，外源 ATP 的处理阻止了 DlAOX1、DlAOX2、DlPUMP3 和 DlPUMP5 表达的增加，有助于维持更高的 ATP、ADP 含量和更高的能量电荷水平，但采后龙眼果实呼吸速率较低，细胞膜通透性较低，果皮褐变指数较低。这些数据表明外源ATP的供应可以减少对DlAOX1、DlAOX2、DlPUMP3和DlPUMP5表达的诱导，从而减少能量耗散并延缓能量水平的下降，从而导致龙眼采后延迟衰老。