Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins based on metabolite profiling analysis.,Journal of Pharmaceutical and Biomedical Analysis

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Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins based on metabolite profiling analysis.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2020-02-27 , DOI: 10.1016/j.jpba.2020.113212
Chao-Geng Lyu ₁ , Chuan-Zhi Kang ₁ , Li-Ping Kang ₁ , Jian Yang ₁ , Sheng Wang ₁ , Ya-Li He ₁ , Ai-Ping Deng ₁ , Hong-Yang Wang ₁ , Lu-Qi Huang ₁ , Lan-Ping Guo ₁

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The root tuber of Ophiopogon japonicus (Thunb.) Ker-Gawl ("Maidong" in Chinese), with steroidal saponins and homoisoflavonoids as its representative chemical compositions, is a representative medicinal herbs with multiple major producing areas. This study aimed to distinguish the O. japonicas samples from Zhejiang and Sichuan by using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS)-based metabolome analysis. Firstly, a global chemical constituent identification of O. japonicas was carried out by using both automatic and manual methods. An integrated steroidal saponins structural identification strategy in O. japonicas based on exact mass information, fragmentation characteristics and retention time was developed. Overall, 135 steroidal saponins, 47 homoisoflavonoids and 9 other metabolites were quickly identified or tentatively identified from the MSE continuum data. Furthermore, multivariate statistical analysis revealed that O. japonicas from Zhejiang and Sichuan can clearly be separated and some markers were screened. Moreover, some major active components including total soluble sugar, total soluble polyphenol, total flavonoid, total saponin and 10 specific compounds were analyzed quantitatively. In general, these results showed that there were many differences between the metabolic profile data of O. japonicas from different producing areas, O. japonicas from Sichuan showed higher level steroidal saponins and samples from Zhejiang had higher contents of homoisoflavonoids specifically, and indicated that metabolite profiling by UPLC/Q-TOF MS is an effective approach for the discrimination of medicinal herbs from different geographical origins.

中文翻译：

基于代谢物谱分析的不同地理来源的麦冬的结构特征和鉴别。

麦冬的块根是具有代表性的药用植物，具有多个主要产区，甾体皂苷和同型异黄酮是其主要化学成分。本研究旨在通过使用超高效液相色谱与基于四极杆飞行时间质谱（UPLC / Q-TOF MS）的代谢组分析来区分浙江和四川的粳稻样品。首先，通过自动和手动两种方法对日本粳稻进行了全球化学成分鉴定。基于准确的质量信息，片段化特征和保留时间，开发了一种综合的甾体皂苷结构鉴定策略。总共135种甾体皂苷，从MSE连续数据中可以快速鉴定或初步鉴定出47种异黄酮和9种其他代谢物。此外，多元统计分析表明，浙江和四川的粳稻可以清楚地分离，并筛选出一些标记。此外，定量分析了一些主要活性成分，包括总可溶性糖，总可溶性多酚，总黄酮，总皂苷和10种特定化合物。总体而言，这些结果表明，不同产地的粳稻的代谢谱数据之间存在许多差异，四川的粳稻显示甾体皂苷含量较高，而浙江的样本中的异黄酮含量更高，

更新日期：2020-02-27

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