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Visualizing the Role of Boundary Elements in Enhancer-Promoter Communication.
Molecular Cell ( IF 14.5 ) Pub Date : 2020-02-27 , DOI: 10.1016/j.molcel.2020.02.007
Moe Yokoshi 1 , Kazuma Segawa 2 , Takashi Fukaya 3
Affiliation  

Formation of self-associating loop domains is a fundamental organizational feature of metazoan genomes. Here, we employed quantitative live-imaging methods to visualize impacts of higher-order chromosome topology on enhancer-promoter communication in developing Drosophila embryos. Evidence is provided that distal enhancers effectively produce transcriptional bursting from target promoters over distances when they are flanked with boundary elements. Importantly, neither inversion nor deletion of a boundary element abrogates this "enhancer-assisting activity," suggesting that they can facilitate intra-domain enhancer-promoter interaction and production of transcriptional bursting independently of topologically associating domain (TAD) formation. In contrast, domain-skipping activity of distal enhancers was lost after disruption of topological domains. This observation raises a possibility that intra-domain and inter-domain enhancer-promoter interactions are differentially regulated by chromosome topology.

中文翻译:

可视化边界元素在增强子-促进子通信中的作用。

自缔合环域的形成是后生动物基因组的基本组织特征。在这里,我们采用定量实时成像方法来可视化高阶染色体拓扑对果蝇胚胎发育中的增强子-启动子通讯的影响。提供的证据表明,当远端启动子与边界元件侧接时,远距离增强子可有效地从远距离启动子产生转录爆发。重要的是,边界元素的倒置或缺失都不能消除这种“增强子辅助活性”,这表明它们可以促进域内增强子与启动子的相互作用以及转录爆发的产生,而与拓扑关联域(TAD)的形成无关。相反,拓扑结构域破坏后,远端增强子的结构域跳过活动丢失。该观察结果提出了域内和域间增强子-启动子相互作用受染色体拓扑差异调节的可能性。
更新日期:2020-02-27
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