The extraction of collagen from fish skins is being proposed as strategy for valorization of marine origin by-products, being a sustainable alternative to mammal collagen. The method commonly uses solutions of organic acids, but new methodologies are arising, aiming to improve process yields and/or the properties of the resulting products. In this work, skins removed from salt brine Atlantic cod (Gadus morhua) were used to extract collagen, using water acidified with CO₂, obtaining an extraction yield of 13.8% (w/w). Acidified water extracted collagen (AWC) presented a total content of proline-like amino acids of 151/1000 residues, with a degree of hydroxylation of 38%, and its SDS-PAGE profile is compatible with type I collagen. Moreover, FTIR, CD and XRD results suggest the presence of preserved triple helix, having a denaturation temperature of 32.3 °C as determined by micro-DSC. AWC exhibited a typical shear thinning behavior, interesting regarding their further processing, namely in jelly-like formulations. Additionally, the presence of AWC in MRC-5 human fibroblasts culture did not affect cell viability, demonstrating the non-cytotoxic behavior. Overall, the results support the efficiency of the proposed approach for collagen extraction and further enable the design of methodologies to address AWC use in biomedical or cosmetic context.

提议从鱼皮中提取胶原蛋白作为海洋来源副产品的增值策略，是哺乳动物胶原蛋白的可持续替代品。该方法通常使用有机酸的溶液，但是出现了新的方法，旨在提高工艺产量和/或所得产物的性能。在这项工作中，从盐水大西洋大西洋鳕鱼（Gadus morhua）中去除的皮被用于提取胶原蛋白，并用CO ₂酸化了水，提取率为13.8％（w/ w）。酸化水提取胶原蛋白（AWC）的总脯氨酸样氨基酸含量为151/1000个残基，羟化度为38％，其SDS-PAGE谱与I型胶原蛋白相容。此外，FTIR，CD和XRD结果表明存在保留的三重螺旋，通过微DSC测定，变性温度为32.3°C。AWC表现出典型的剪切稀化行为，对其进一步加工（即在果冻状配方中）感兴趣。另外，MRC-5人成纤维细胞培养物中AWC的存在不影响细胞生存力，证明了其非细胞毒性行为。总体而言，研究结果支持了所提出的胶原蛋白提取方法的效率，并进一步使方法设计能够解决在生物医学或化妆品领域使用AWC的问题。