Plant diseases caused by pathogenic microorganisms represent a serious threat to plant productivity, food security, and natural ecosystems. An effective framework for early warning and rapid response is a crucial element to mitigate or prevent the impacts of biological invasions of plant pathogens. For these reasons, detection tools play an important role in monitoring plant health, surveillance, and quantitative pathogen risk assessment, thus improving best practices to mitigate and prevent microbial threats. The need to reduce the time of diagnosis has prompted plant pathologists to move towards more sensitive and rapid methods such as molecular techniques. Considering prevention to be the best strategy to protect plants from diseases, this review focuses on fast and reliable molecular methods to detect the presence of woody plant pathogens at early stage of disease development before symptoms occur in the host. A harmonized pool of novel technical, methodological, and conceptual solutions is needed to prevent entry and establishment of new diseases in a country and mitigate the impact of both invasive and indigenous organisms to agricultural and forest ecosystem biodiversity and productivity.

中文翻译：

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快速可靠的分子方法检测木本植物中的真菌病原体。


由病原微生物引起的植物病害对植物生产力、粮食安全和自然生态系统构成严重威胁。有效的早期预警和快速反应框架是减轻或防止植物病原体生物入侵影响的关键因素。由于这些原因，检测工具在监测植物健康、监测和定量病原体风险评估方面发挥着重要作用，从而改进减轻和预防微生物威胁的最佳实践。减少诊断时间的需要促使植物病理学家转向更灵敏和快速的方法，例如分子技术。考虑到预防是保护植物免受疾病侵害的最佳策略，本综述重点关注快速可靠的分子方法，以在宿主出现症状之前的疾病发展早期阶段检测木本植物病原体的存在。需要一个协调一致的新技术、方法和概念解决方案，以防止新疾病进入一个国家并在那里定居，并减轻入侵生物和本土生物对农业和森林生态系统生物多样性和生产力的影响。