Fighting against Fast Speckle Decorrelation for Light Focusing inside Live Tissue by Photon Frequency Shifting,ACS Photonics

当前位置： X-MOL 学术 › ACS Photonics › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fighting against Fast Speckle Decorrelation for Light Focusing inside Live Tissue by Photon Frequency Shifting
ACS Photonics ( IF 6.5 ) Pub Date : 2020-03-09 , DOI: 10.1021/acsphotonics.0c00027
Jiamiao Yang ₁ , Lei Li ₁ , Jingwei Li ₁ , Zhongtao Cheng ₁ , Yan Liu ₁ , Lihong V. Wang ₁

Affiliation

Light focusing inside live tissue by digital optical phase conjugation (DOPC) has drawn increasing interest due to its potential biomedical applications in optogenetics, microsurgery, phototherapy, and deep-tissue imaging. However, fast physiological motions in a live animal, including blood flow and respiratory motions, produce undesired photon perturbation and thus inevitably deteriorate the performance of light focusing. Here, we develop a photon-frequency-shifting DOPC method to fight against fast physiological motions by switching the states of a guide star at a distinctive frequency. Therefore, the photons tagged by the guide star are well detected at the specific frequency, separating them from the photons perturbed by fast motions. Light focusing was demonstrated in both phantoms in vitro and mice in vivo with substantially improved focusing contrast. This work puts a new perspective on light focusing inside live tissue and promises wide biomedical applications.

中文翻译：

对抗快速散斑去相关以通过光子频移将光聚焦在活组织内部

由于其在光遗传学，显微外科，光疗和深层组织成像中的潜在生物医学应用，通过数字光学相位共轭（DOPC）聚焦在活组织内部的光引起了越来越多的兴趣。但是，活体动物的快速生理运动，包括血流和呼吸运动，会产生不希望的光子扰动，因此不可避免地会降低聚光性能。在这里，我们开发了一种光子频移DOPC方法，通过以不同的频率切换引导星的状态来对抗快速的生理运动。因此，在特定频率下可以很好地检测到由引导星标记的光子，从而将它们与被快速运动扰动的光子分开。在体模的体外和体内的小鼠中都证明了光聚焦，聚焦对比度大大提高。这项工作为聚焦在活组织内部的光提供了新的视角，并有望在生物医学领域得到广泛应用。

更新日期：2020-03-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11