Abstract To improve simultaneous characterization of structural and compositional features of infant formula powder the objectives of this study were to: 1) advance micro and macroscale fluorescence imaging methods to characterize the distribution of sugars, proteins and lipids in infant formula powder and 2) understand the impact of processing conditions and storage on the microstructure of infant formula powder. For micro and macroscale fluorescence imaging of infant formula, the samples were pre-stained with a trace amount of fluorescently labeled milk proteins, a fluorescent analogue of a simple sugar and a lipid specific chemical stain prior to spray drying. The labeled samples of infant formula powder from evaporator, collection chamber and cyclone units of a spray dryer were collected and analyzed using particle size measurements, scanning electron microscopy, confocal laser microscopy and widefield fluorescence macroscopy. In addition, the powder samples were also analyzed after storage for 6 months. The results of this study demonstrate that pre-staining of the infant formula powder eliminates the potential artifacts introduced by post-staining labeling of the infant formula powders using solvents. The results demonstrate that significant (p

中文翻译：

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用于同时映射婴儿配方奶粉中多种成分分布的多尺度成像方法

摘要 为了同时表征婴儿配方奶粉的结构和成分特征，本研究的目标是：1) 推进微观和宏观荧光成像方法来表征婴儿配方奶粉中糖、蛋白质和脂质的分布，以及 2) 了解加工条件和储存对婴幼儿配方粉微观结构的影响。对于婴儿配方奶粉的微观和宏观荧光成像，样品在喷雾干燥之前用痕量荧光标记的牛奶蛋白、单糖的荧光类似物和脂质特异性化学染色剂进行预染色。收集来自喷雾干燥器的蒸发器、收集室和旋风单元的婴儿配方奶粉标记样品，并使用粒度测量进行分析，扫描电子显微镜、共聚焦激光显微镜和宽场荧光显微镜。此外，粉末样品在储存 6 个月后也进行了分析。该研究的结果表明，婴儿配方奶粉的预染色消除了使用溶剂对婴儿配方奶粉进行后染色标记所引入的潜在伪影。结果表明显着 (p