The current study was conducted to develop a quantitative polymerase chain reaction (qPCR) assay for Bifidobacterium animalis ssp. lactis BB-12 quantification in microcapsules matrix with full-fat goat milk and inulin-type fructans. DNA was isolated from milk, feed solutions (before spray drying) and microcapsules (after spray drying) using DNAzol. Two primer pairs targeting Bal-23S or Tuf sequences were evaluated by qPCR. The qPCR efficiency was higher (89.5%) using the Tuf primers than Bal-23S primers (84.8%). Tuf primer pair was able to selectively detect B. animalis ssp. lactis BB-12. After, quantification of bifidobacteria in the microcapsules matrix by Tuf qPCR assay was compared to conventional enumeration by plate counting. The analysis of probiotic feed solutions and microcapsules showed higher (P < 0.05) bacterial enumeration determined by Tuf qPCR assay compared to those obtained by plate counting. This qPCR assay was considered a rapid and sensitive alternative for the quantification of B. animalis ssp. lactis BB-12 in probiotic microcapsules compared to plate counting.

进行当前的研究以开发用于动物双歧杆菌ssp的定量聚合酶链反应（qPCR）测定。全脂山羊奶和菊粉型果聚糖在微胶囊基质中对乳酸BB-12的定量分析。使用DNAzol从牛奶，进料溶液（喷雾干燥前）和微囊（喷雾干燥后）中分离DNA。通过qPCR评估靶向Bal-23S或Tuf序列的两个引物对。使用Tuf引物的qPCR效率更高（89.5％），而与Bal-23S引物（84.8％）相比更高。Tuf引物对能够选择性检测动物双歧杆菌。乳酸菌BB-12。之后，将通过Tuf qPCR测定的微胶囊基质中双歧杆菌的定量与通过板计数的常规计数进行比较。益生菌饲料溶液和微胶囊的分析显示，与通过平板计数获得的细菌计数相比，通过Tuf qPCR分析确定的细菌计数更高（P <0.05）。该qPCR测定法被认为是量化B. animalis ssp的快速灵敏方法。与平板计数相比，益生菌微胶囊中的乳酸菌BB-12。