A wide-scope analytical method was developed and validated for the determination of tropane alkaloids (TAs) in honey samples. A simple and fast extraction procedure, using a mixture of methanol/water/formic acid (75/25/0.1, v/v/v) as extraction solvent, followed by a clean-up with graphitized black carbon (GBC) and magnesium sulphate was optimized, and compounds were analysed by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS-Orbitrap). Validation of the proposed method provided adequate linearity (R²>0.99), trueness (recoveries 71-120%) and precision (relative standard deviation, RSD≤20.1%), with limits of quantitation (LOQs) at 20 µg/kg (except anisodamine and scopolamine at 40 µg/kg) and a significant matrix effect (≤-50%). Nineteen honey samples were analysed, but only one was positive, containing 27 µg/kg of scopolamine. Additionally, a post-targeted screening was performed, and 47% of samples were contaminated with different herbicides, insecticides and veterinary drugs. Therefore, the proposed analytical method is a powerful tool for both targeted TAs and post-targeted contaminant analysis.

开发了一种广谱分析方法，并已用于测定蜂蜜样品中的托烷生物碱（TAs）。使用甲醇/水/甲酸（75/25 / 0.1，v / v / v）的混合物作为萃取溶剂的简单且快速的萃取程序，然后用石墨化黑碳（GBC）和硫酸镁进行净化对化合物进行了优化，并通过液相色谱与高分辨率质谱法（LC-HRMS-Orbitrap）进行了分析。所提出方法的验证提供了足够的线性度（R ²> 0.99），真实度（回收率71-120％）和精密度（相对标准偏差，RSD≤20.1％），定量限（LOQ）为20 µg / kg（山iso碱和东pol碱除外，均为40 µg / kg），并且显着的基质效应（≤-50％）。分析了19个蜂蜜样品，但只有一个阳性，含量为27 µg / kg东pol碱。此外，进行了靶向后筛选，并且47％的样品被不同的除草剂，杀虫剂和兽药污染。因此，所提出的分析方法是针对目标TA和目标后污染物分析的强大工具。