Abstract Imaging analysis, especially bioimaging analysis, has been a hot research topic in recent years. There are numerous imaging analysis techniques for diverse applications of a wide spectrum of samples, with their unique advantages and disadvantages, and there are several related reviews published yearly. Among them, imaging mass spectrometry (IMS) is a relatively novel analytical technique for studying the distribution of molecular or ionic species at the level of tissue, cell, or subcellular, with its main feature of combining mass spectra for molecular identification and image visualization for quick and convenient analysis. The IMS does not require chemical labeling or complex sample preparation. This review, therefore, mainly focuses on the popular emerging IMS technique, including related ionization techniques in connection with their IMS applications, and some unique optical imaging techniques such as chemiluminescence imaging and dual-modal bioimaging for biological sample analysis, with 105 related recent references.

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用于生物样品成像分析的质谱/光谱分析技术简述

摘要 成像分析，尤其是生物成像分析，是近年来的研究热点。有许多成像分析技术可用于广泛样本的不同应用，具有独特的优点和缺点，并且每年都会发表几篇相关评论。其中，成像质谱（IMS）是一种相对较新的分析技术，用于在组织、细胞或亚细胞水平上研究分子或离子物质的分布，其主要特点是将质谱进行分子识别和图像可视化相结合，快速方便的分析。IMS 不需要化学标记或复杂的样品制备。因此，本综述主要关注流行的新兴 IMS 技术，