ABSTRACT Microfluidic paper-based analytical devices (µPADs) have attracted much attention over the past decade because they offer clinicians the ability to deliver point-of-care testing and onsite analysis. Many of the advantages of µPADs, however, are limited to work in a laboratory setting due to the difficulties of processing data when using electronic devices in the field. This review focuses on the use of µPADs that have the potential to work without batteries or with only small and portable devices such as smartphones, timers, or miniaturized detectors. The µPADs that can be operated without batteries are, in general, those that allow the visual judgment of analyte concentrations via readouts that are measured in time, distance, count, or text. Conversely, a smartphone works as a camera to permit the capture and processing of an image that digitizes the color intensity produced by the reaction of an analyte with a colorimetric reagent. Miniaturized detectors for electrochemical, fluorometric, chemiluminescence, and electrochemiluminescence methods are also discussed, although some of them require the use of a laptop computer for operation and data processing.

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具有免仪器检测和小型化便携式检测器的微流体纸基分析设备

摘要 微流体纸基分析设备 (μPAD) 在过去十年中引起了很多关注，因为它们为临床医生提供了提供即时检测和现场分析的能力。然而，由于在现场使用电子设备时处理数据的困难，μPAD 的许多优点仅限于在实验室环境中工作。本评论重点介绍了 µPAD 的使用，这些 µPAD 有可能在没有电池的情况下工作，或仅与智能手机、计时器或小型化探测器等小型便携式设备配合使用。可以在没有电池的情况下运行的 µPAD 通常允许通过以时间、距离、计数或文本形式测量的读数对分析物浓度进行视觉判断。反过来，智能手机作为相机可以捕获和处理图像，该图像将分析物与比色试剂反应产生的颜色强度数字化。还讨论了用于电化学、荧光、化学发光和电化学发光方法的小型化检测器，尽管其中一些需要使用膝上型计算机进行操作和数据处理。