Abstract Raman spectroscopy can detect conformational changes in collagen structures and these findings are reviewed in this article. More specifically, some progressive diseases are mainly caused by alterations of collagen molecules but what is occurring at the biochemical level of this complex molecule usually remains unclear. While it may be true that a number of analytical techniques can analyze collagen, most of them have a series of limitations that limit their applicability to a wide range of samples. To understand in more detail the progression of a disease due to changes in the collagen structure, a technique that can detect subtle alterations at the biochemical level is needed. Raman spectroscopy is a label-free and noninvasive technique that can easily pick up on any conformational changes reflected primarily at the lipids, amides and proline and hydroxyproline regions. This review is the first compilation of studies of conformational changes in collagen molecules, providing help to understand changes in collagen biochemistry that can be of relevance to the human wound healing, ageing and pathologies.

中文翻译：

---

用拉曼光谱表征胶原蛋白的结构变化

摘要 拉曼光谱可以检测胶原结构的构象变化，本文对这些发现进行了综述。更具体地说，一些进行性疾病主要是由胶原蛋白分子的改变引起的，但在这种复杂分子的生化水平上发生了什么通常仍不清楚。虽然确实有许多分析技术可以分析胶原蛋白，但它们中的大多数都有一系列限制，限制了它们对广泛样品的适用性。为了更详细地了解由于胶原结构变化引起的疾病进展，需要一种可以检测生化水平细微变化的技术。拉曼光谱是一种无标记和非侵入性技术，可以轻松识别主要反映在脂质上的任何构象变化，酰胺和脯氨酸和羟脯氨酸区域。这篇综述是对胶原蛋白分子构象变化研究的第一次汇编，有助于了解可能与人类伤口愈合、衰老和病理相关的胶原蛋白生物化学变化。