Pluripotent cells transiently develop during peri-implantation embryogenesis and have the capacity to convert into three embryonic lineages. Two typical states of pluripotency, naïve and primed, can be experimentally induced in vitro. The in vitro naïve state can be stabilized in response to environmental inductive cues via a unique transcriptional regulatory program. However, interference with various signaling pathways creates a spectrum of alternative pluripotent cells that display different functions and molecular expression patterns. Similarly, human naïve pluripotent cells can be placed into two main levels - intermediate and bona fide. Here, we discuss several culture conditions that have been used to establish naïve-associated gene regulatory networks in human pluripotent cells. We also describe different transcriptional patterns in various culture systems that are associated with these two levels of human naïve pluripotency.

中文翻译：

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人类幼稚多能性的信号调节剂。

多能细胞在植入前后的胚胎发生过程中短暂发育，并具有转化为三个胚胎谱系的能力。可以通过体外实验诱导幼稚和已准备好两种典型的多能性状态。通过独特的转录调控程序，可以响应于环境诱导的信号来稳定体外的幼稚状态。然而，对各种信号传导途径的干扰产生了显示不同功能和分子表达模式的多种多能细胞。同样，人类幼稚多能细胞可分为两个主要水平：中间水平和真正水平。在这里，我们讨论了几种培养条件，这些条件已用于在人类多能细胞中建立幼稚相关的基因调控网络。