Click Chemistry-Assisted Bioconjugates for Hapten Immunodiagnostics.,Bioconjugate Chemistry

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Click Chemistry-Assisted Bioconjugates for Hapten Immunodiagnostics.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-02-25 , DOI: 10.1021/acs.bioconjchem.0c00099
Daniel López-Puertollano ₁ , Consuelo Agulló ₁ , Josep V Mercader ₂ , Antonio Abad-Somovilla ₁ , Antonio Abad-Fuentes ₂

Affiliation

Bioorthogonal reactions have revolutionized the way low-molecular-weight compounds are coupled to biomolecules. Organic chemistry, polymer science, and chemical biology are among the disciplines that have benefited the most from this breakthrough. Despite the reliability of the click chemistry concept for the efficient and chemoselective functionalization of biomacromolecules with haptens at preferred positions, the fact that azide-alkyne cycloaddition reactions originate new chemical moieties as part of the linker may have delayed their application in the immunodiagnostic field. Using the mycotoxin ochratoxin A as a model compound, we herein demonstrate for the first time that bioconjugates arising from the ligation between an azido-bearing hapten and an alkyne-modified carrier protein are able to elicit the generation of high-affinity monoclonal antibodies suitable for the development of rapid methods for the immunodetection of small organic molecules.

中文翻译：

单击用于半抗原免疫诊断的化学辅助生物缀合物。

生物正交反应彻底改变了低分子量化合物与生物分子偶联的方式。有机化学，高分子科学和化学生物学是从该突破中受益最大的学科。尽管点击化学概念对于在优选位置具有半抗原的生物大分子的高效和化学选择性功能化具有可靠性，但叠氮化物-炔烃环加成反应产生新的化学部分这一事实作为连接子的一部分可能会延迟其在免疫诊断领域的应用。使用霉菌毒素曲霉毒素A作为模型化合物，

更新日期：2020-03-03

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