As an important organelle, the endoplasmic reticulum (ER) participates in the synthesis and secretion of various proteins, glycogen, lipids and cholesterol in eukaryotic cells. In this work, an endoplasmic reticulum-targeted reversible fluorescent probe (ER-Se) was designed and synthesized. The probe, based on a selenide group, shows high sensitivity and good selectivity toward HClO (LOD = 0.85 μM). In addition, the probe has reversible capability towards HClO/GSH. Most importantly, co-location experiment results indicated that the probe exhibited a great ability to target the endoplasmic reticulum. Furthermore, the probe was successfully applied to detect exogenous and endogenous HClO in ER and monitored the redox status changes during ER stress.

中文翻译：

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用硒荧光探针揭示内质网中的氧化还原状态

内质网（ER）作为重要的细胞器，参与真核细胞中各种蛋白质，糖原，脂质和胆固醇的合成和分泌。在这项工作中，设计并合成了内质网靶向可逆荧光探针（ER-Se）。基于硒化物基团的探针显示出高灵敏度和对HClO的良好选择性（LOD = 0.85μM）。另外，该探针对HClO / GSH具有可逆性。最重要的是，共置位实验结果表明该探针表现出强大的靶向内质网的能力。此外，该探针已成功应用于检测内质网中的内源性和内源性HClO，并监测内质网应激期间氧化还原状态的变化。