Abstract Pressure-assisted electrokinetic injection (PAEKI) as the online method was developed for the simultaneous enrichment of six sulfonamides (SAs), including sulfamethazine (SMZ), sulfamerazine (SMR), sulfamonomethoxine (SMM), sulfadizine (SDZ), sulfamethoxazole (SMX) and sulfacetamide (SFA) in different animal husbandry products and environmental waters samples, followed by capillary zone electrophoresis (CZE) determination. Various parameters affecting the separation performance of CZE and the enrichment efficiency of PAEKI were optimized in detail. Under optimal conditions, the six SAs were completely separated within 8.5 min, and the enrichment factors for SMZ, SMR, SMM, SDZ, SMX and SFA were 39, 47, 53, 50, 54 and 62 folds, respectively, compared with the direct CZE. The limits of detection (LOD) ranged from 0.0018–0.0163 μg/mL, 0.0083–0.0638 μg/mL and 0.0052–0.0478 μg/mL in milk, pork and egg samples, respectively, as well as limits of quantification (LOQ) within 0.0061–0.0503 μg/mL, 0.0253–0.1826 μg/mL and 0.0164–0.1475 μg/mL, respectively. Tap water, lake water and seawater were also examined for investigation of applicability of the proposed method. Satisfactory recoveries in the range of 89–113 % were obtained with the three spiked food samples, and the relative standard deviations were from 1.6 to 7.3%. Simple and effective online enrichment method provides a promising perspective for CZE measurements of SAs in complex matrices with high accuracy, sensitivity and rapidity.

中文翻译：

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压力辅助电动注射结合毛细管区带电泳同时富集/测定畜牧产品和环境水中的六种磺胺类药物

摘要 开发了压力辅助电动注射 (PAEKI) 作为在线方法同时富集六种磺胺类药物 (SA)，包括磺胺二甲嘧啶 (SMZ)、磺胺二甲嘧啶 (SMR)、磺胺间甲氧嘧啶 (SMM)、磺胺嘧啶 (SDZ)、磺胺甲恶唑 (SMX) ) 和磺胺醋酰 (SFA) 在不同畜牧产品和环境水样中，然后进行毛细管区带电泳 (CZE) 测定。对影响CZE分离性能和PAEKI富集效率的各种参数进行了详细优化。在最佳条件下，6种SAs在8.5 min内完全分离，SMZ、SMR、SMM、SDZ、SMX和SFA的富集因子分别为39、47、53、50、54和62倍。捷克。检测限 (LOD) 范围为 0.0018–0.0163 μg/mL，0. 牛奶、猪肉和鸡蛋样品中的浓度分别为 0083–0.0638 µg/mL 和 0.0052–0.0478 µg/mL，定量限 (LOQ) 分别为 0.0061–0.0503 µg/mL、0.0253–0.1826 µg/mL 和 50-50 µg/mL微克/毫升，分别。还检查了自来水、湖水和海水，以研究所提出方法的适用性。三种加标食品样品获得了 89% 至 113% 范围内的令人满意的回收率，相对标准偏差为 1.6% 至 7.3%。简单有效的在线富集方法为复杂基质中 SA 的 CZE 测量提供了一个有前景的前景，具有高精度、灵敏度和快速性。分别。还检查了自来水、湖水和海水，以研究所提出方法的适用性。三种加标食品样品获得了 89% 至 113% 范围内的令人满意的回收率，相对标准偏差为 1.6% 至 7.3%。简单有效的在线富集方法为复杂基质中 SA 的 CZE 测量提供了一个有前景的前景，具有高精度、灵敏度和快速性。分别。还检查了自来水、湖水和海水，以研究所提出方法的适用性。三种加标食品样品获得了 89% 至 113% 范围内的令人满意的回收率，相对标准偏差为 1.6% 至 7.3%。简单有效的在线富集方法为复杂基质中 SA 的 CZE 测量提供了一个有前景的前景，具有高精度、灵敏度和快速性。