UV exposure could induce carcinogenic mutation in the human cells, including CPD (Cyclobutane pyrimidine dimer), and 6–4 pp (6–4 photoproduct) DNA damages. Spiting the active Base excision repair (BER) system of human cells, it lacks initiator glycosylase, rendering these damages to be only repaired through Nucleotide excision repair (NER) system. Some microorganisms such as Deinococcus radiodurans bacteria have a BER system for repairing these damages with an enzyme coded by the uvsE gene. This study evaluated the efficacy of the recombinant UVSE protein for repairing the CPD and 6–4 pp DNA damages in human cells. At the current study, the optimized sequence of the uvsE gene was synthesized and expressed in Hek293T cell line. The identity of protein was ascertained through ELISA assay and the stability of expression was measured via qPCR. The human Hek293T cells with the recombinant protein and without it were exposed to the UV light, and the repair of DNA damages was analyzed in both conditions using CPD and 6–4PP ELISA Combo Kit. The results indicated that uvsE gene was successfully colonized and expressed and expression showed to be stable. Hek293T cells with recombinant uvsE gene showed efficacy at repairing 80% of CPD and 85% of 6–4 photoproducts during one hour, and more than 95% over 4 h' repair time. Considering the outcome of this study, it could be concluded that the uvsE recombinant product is highly efficient at repairing both CPD and 6–4 pp damages and could be considered as a preventive agent for UV-induced skin cancers.

紫外线照射可能导致人体细胞发生致癌突变，包括CPD（环丁烷嘧啶二聚体）和6–4 pp（6–4光产物）DNA损伤。喷洒人细胞的主动碱基切除修复（BER）系统后，它缺少引发剂糖基化酶，致使这些损伤只能通过核苷酸切除修复（NER）系统修复。某些微生物，例如放射性双歧球菌细菌具有BER系统，可使用uvsE基因编码的酶修复这些损伤。这项研究评估了重组UVSE蛋白修复人细胞中CPD和6–4 pp DNA损伤的功效。在当前研究中，合成了uvsE基因的优化序列，并在Hek293T细胞系中表达。通过ELISA测定确定蛋白质的身份，并通过qPCR测量表达的稳定性。将含有重组蛋白而不含有重组蛋白的人Hek293T细胞暴露于紫外线下，并使用CPD和6-4PP ELISA Combo Kit在两种条件下分析DNA损伤的修复。结果表明，uvsE基因已成功克隆并表达，表达稳定。具有重组uvsE基因的Hek293T细胞在一小时内可修复80％的CPD和85％的6–4个光产品，在4小时的修复时间内可修复95％以上的功效。考虑到这项研究的结果，可以得出结论，uvsE重组产品在修复CPD和6–4 pp损伤方面非常有效，可以被认为是紫外线诱发的皮肤癌的预防剂。