We report herein the use of nanofibrillated cellulose (NFC) for development of enzyme assemblies in an oriented manner for biotransformation with in situ cofactor regeneration. This is achieved by developing fusion protein enzymes with cellulose-specific binding domains. Specifically, lactate dehydrogenase and NADH oxidase were fused with a cellulose binding domain, which enabled both enzyme recovery and assembling in essentially one single step by using NFC. Results showed that the binding capacity of the enzymes was as high as 0.9 μmol-enzyme/g-NFC. Compared to native parent free enzymes, NFC-enzyme assemblies improved the catalytic efficiency of the coupled reaction system by over 100%. The lifetime of enzymes was also improved by as high as 27 folds. The work demonstrates promising potential of using biocompatible and environmentally benign bio-based nanomaterials for construction of efficient catalysts for intensified bioprocessing and biotransformation applications.

我们在此报告了纳米原纤化纤维素（NFC）在定向组装酶的过程中用于原位辅因子再生生物转化的发展。这是通过开发具有纤维素特异性结合域的融合蛋白酶来实现的。具体而言，将乳酸脱氢酶和NADH氧化酶与纤维素结合域融合，这使得通过使用NFC基本上可以在一个步骤中进行酶的回收和组装。结果表明，这些酶的结合能力高达0.9μmol酶/ g-NFC。与天然亲本游离酶相比，NFC酶组件将偶联反应系统的催化效率提高了100％以上。酶的寿命也提高了27倍。