Oxidative stress can play an important role in the autoxidation of glucose, glycation of proteins and activation of polyol metabolism, and the development of various complications in diabetes. This study investigated the protective effect of Paliurus spina-christi (PSC) fruit against diabetes-induced pathologies such as inflammation caused by oxidative stress and its phytochemical content. The bioactive compounds of Paliurus spina-christi fruit extract (PSC-FE) prepared by the infusion method were determined by LC-MS/MS analysis, and the metal reduction and radical removal activity were determined in vitro by DPPH, ABTS, FRAP and CUPRAC methods. The effect of PSC-FE on catalase and superoxide dismutase enzymes and lipid peroxidation in streptozotocin-induced diabetic rat tissues was determined in vivo. For determining in vivo antioxidant activity, the study was carried out on 30 Wistar albino male rats that were distributed into 3 research and 2 control groups. As a result of LC-MS/MS analysis, malic acid (28,424.18 ± 3.21 μg/g PSC-FE) and rutin (23,459.68 ± 3.73 μg/g PSC-FE) are the highest among the 22 phenolic compounds. The PSC fruit water extracts were determined to have a more powerful antioxidant activity than standard antioxidants. In those groups that received PSC-FE, catalase and superoxide dismutase enzyme levels that decreased in patient group were found to have a statistically meaningful increase in treatment groups (P < 0.01). Although malondialdehyde amount, the last product of lipid peroxidation, was observed to have a statistically meaningful increase in patient group when compared with control group, this increase was decreased in all treatment groups in a statistically meaningful manner (P < 0.01). The PSC was seen to be more successful, when compared with standard antioxidants. The PSC-FE was found to have a rich phenolic diversity. It can be said that the PSC may have a significant therapeutic effect on diabetes caused by oxidative stress due to its biological activities and content.

氧化应激可在葡萄糖的自氧化，蛋白质的糖基化和多元醇代谢的活化以及糖尿病各种并发症的发生中起重要作用。这项研究调查了刺桐-克里斯蒂（PSC）水果对糖尿病引起的病理学的保护作用，例如氧化应激引起的炎症及其植物化学成分。棘柳克里斯蒂的生物活性化合物采用LC-MS / MS分析法测定了浸提法制得的水果提取物（PSC-FE），并通过DPPH，ABTS，FRAP和CUPRAC法测定了金属还原和自由基去除活性。在体内确定了PSC-FE对链脲佐菌素诱导的糖尿病大鼠组织中过氧化氢酶和超氧化物歧化酶和脂质过氧化的影响。为了确定体内抗氧化活性，在30只Wistar白化病雄性大鼠上进行了研究，将其分为3个研究组和2个对照组。LC-MS / MS分析的结果是，在22种酚类化合物中，苹果酸（28,424.18±3.21μg/ g PSC-FE）和芦丁（23,459.68±3.73μg/ g PSC-FE）最高。经测定，PSC水果水提取物比标准抗氧化剂具有更强大的抗氧化剂活性。在收到PSC-FE的人群中，P  <0.01）。尽管与对照组相比，患者组中丙二醛含量（脂质过氧化的最后产物）在统计学上具有有意义的增加，但在所有治疗组中这种增加均以统计学上有意义的方式减少（P  <0.01）。与标准抗氧化剂相比，PSC被认为更成功。发现PSC-FE具有丰富的酚类多样性。可以说，PSC由于其生物活性和含量而对氧化应激引起的糖尿病具有重要的治疗作用。