Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system,Biotechnology and Applied Biochemistry

当前位置： X-MOL 学术 › Biotechnol. Appl. Bioc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-02-26 , DOI: 10.1002/bab.1903
Yixiao Fan _{1,

2} , Yingbo Li ₁ , Qingfen Liu ₁

Affiliation

An efficient method for the enzymatic synthesis of cephalexin (CEX) from 7‐amino‐3‐deacetoxycephalosporanic acid (7‐ADCA) and d‐phenylglycine methyl ester (PGME) using immobilized penicillin G acylase (IPGA) as catalyst in a suspension aqueous solution system was developed, where the reactant 7‐ADCA and product CEX are mainly present as solid particles. The effects of key factors on the enzymatic synthesis were investigated. Results showed that continuous feeding of PGME was more efficient for the synthesis of CEX than the batch mode. Under the optimized conditions, the maximum 7‐ADCA conversion ratio of 99.3% and productivity of 200 mmol/L/H were achieved, both of which are much superior to the homogeneous aqueous solution system. Besides, IPGA still retained 95.4% of its initial activity after 10 cycles of enzymatic synthesis, indicating the excellent stability of this approach. The developed approach shows great potential for the industrial production of CEX via an enzyme‐based route.

中文翻译：

悬浮水溶液体系中高效酶促合成头孢氨苄

从7-氨基-3-去乙酰氧基头孢菌酸（7-ADCA）和d酶促合成头孢氨苄（CEX）的有效方法开发了在悬浮水溶液系统中使用固定化的青霉素G酰基转移酶（IPGA）作为催化剂的-苯基甘氨酸甲酯（PGME），其中反应物7-ADCA和产物CEX主要以固体颗粒形式存在。研究了关键因素对酶促合成的影响。结果表明，连续进料PGME对CEX的合成比分批模式更有效。在优化的条件下，最大的7-ADCA转化率达到99.3％，生产率达到200 mmol / L / H，这两者均优于均相水溶液系统。此外，经过10次酶促合成后，IPGA仍保留了其95.4％的初始活性，表明该方法具有出色的稳定性。

更新日期：2020-02-26

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11