There is a lack of comprehensive studies on the seroprevalence of tick-borne pathogens in the Republic of Korea. Therefore, the aim of this study was to investigate the seroprevalences of Anaplasma spp. (A. phagocytophilum/A. platys), Borrelia burgdorferi sensu lato, Babesia gibsoni, Ehrlichia spp. (E. canis/E. ewingii), and Ehrlichia chaffeensis in dogs across the Republic of Korea in 2017 and 2018. A total of 2215 serum samples collected from 938 companion dogs, 969 shelter dogs, and 308 military working dogs were examined using commercial enzyme-linked immunosorbent assay (ELISA) and indirect fluorescence immunoassay (IFA) kits. Data collected for each animal, including breed, sex, age, region, season, and dog type, were used for statistical analysis. The overall seroprevalence was highest for Anaplasma spp. (15.1 %), followed by Ehrlichia spp. (10.3 %), B. burgdorferi sensu lato (6.4 %), E. chaffeensis (2.3 %), and B. gibsoni (1.7 %). One hundred and sixty-one dogs had antibodies against two or three different pathogens. The most common combinations were Anaplasma spp. - Ehrlichia spp. (2.1 %), Anaplasma spp. - E. chaffeensis (1.4 %), and Anaplasma spp. - B. burgdorferi sensu lato (1.2 %). Season was significantly associated with the seroprevalences of B. burgdorferi sensu lato and Ehrlichia spp., with dogs presenting the highest percentage of positive results during summer. Anaplasma spp. and B. gibsoni were significantly more prevalent in the northern and southern regions, respectively. The seroprevalences of Anaplasma spp., B. burgdorferi sensu lato, and Ehrlichia spp. were significantly higher in military working dogs, while the seroprevalence of E. chaffeensis was higher in companion dogs. The current findings are important for future surveillance of canine tick-borne pathogens and designing appropriate approaches for the diagnosis and control of these pathogens in the Republic of Korea.

在大韩民国，关于tick传病原体的血清阳性率缺乏全面的研究。因此，本研究的目的是调查无形体的血清阳性率。（A.吞噬细胞/ A。普拉提斯吉），莱姆病螺旋体意义上拉托，吉氏巴贝斯虫，埃立克体属。（E. canis / E。ewingii）和Ehrlichia chaffeensis2017年和2018年在大韩民国的狗中进行了检测。使用商业酶联免疫吸附测定（ELISA）和间接荧光免疫测定（ IFA）套件。为每种动物收集的数据（包括品种，性别，年龄，区域，季节和狗的类型）用于统计分析。Anaplasma spp的总体血清阳性率最高。（15.1％），其次是埃里希氏菌。（10.3％），博氏疏螺旋意义上拉托（6.4％），恰菲埃里希氏体（2.3％），和B. gibsoni（1.7％）。161只狗具有针对两种或三种不同病原体的抗体。最常见的组合是Anaplasma spp。-埃里希氏菌 （2.1％），无形体属。-Chaffeensis大肠杆菌（1.4％）和Anaplasma spp。-B.burgdorferi sensu lato（1.2％）。季节与B. burgdorferi sensu lato和Ehrlichia spp。的血清阳性率显着相关，在夏季，狗表现出最高的阳性结果百分比。无形体属。和吉布森分别在北部和南部地区更为普遍。Anaplasma spp。，B。burgdorferi sensu lato和Ehrlichia spp的血清阳性率。是在军事工作犬显著较高，而血清阳性率恰菲埃里希氏是伴侣犬高。当前的发现对于将来对犬tick传播的病原体的监视以及在大韩民国设计用于诊断和控制这些病原体的适当方法非常重要。