The research of value-added applications for coffee silverskin (CSS) requires studies to investigate potential bioactive compounds and biological activities in CSS extracts. In this study, different ultrasound‐assisted extraction (UAE) methods have been tested to extract bioactive compounds from CSS. The obtained extracts, were characterized using a new HPLC-MS/MS method to detect and quantify 30 bioactive compounds of 2 classes: alkaloids and polyphenols (including phenolic acids, flavonoids, and secoiridoids). CSS extracts obtained with ethanol/water (70:30) as extraction solvent showed the highest levels (p ≤ 0.05) of bioactive compounds (4.01 ± 0.34 % w/w). High content of caffeine was observed with levels varying from 1.00% to 3.59% of dry weight of extract (dw). 18 phenolic compounds were detected in CSS extracts with caffeoylquinic acids (3-CQA, 5-CQA and 3,5-diCQA) as the most abundant polyphenols (3115.6 µg g^-1 to 5444.0 µg g^-1). This study is also one of the first to characterize in-depth flavonoids in CSS revealing the levels of different flavonoids compounds such as rutin (1.63 – 8.70 µg g^-1), quercetin (1.53 – 2.46 µg g^-1), kaempferol (0.76 – 1.66 µg g^-1) and quercitrin (0.15 – 0.51 µg g^-1). Neuroprotective activity of silverskin extracts against H₂O₂-induced damage was evaluated for the first time suggesting for methanol and ethanol/water (70:30) extracts a potential role as protective agents against neurodegeneration due to their ability to counteract oxidative stress and maintain cell viability. Silverskin extracts were not inhibiting the growth of anyone of the bacterial species included in this study but data obtained by water extract might deserve a deeper future investigation on biofilm-related activities, such as quorum sensing or virulence factors’ expression. From their composition and their evidenced biological activities, CSS extracts could represent valuable ingredients in nutraceutical formulations.

对咖啡银皮（CSS）的增值应用的研究要求进行研究，以研究CSS提取物中潜在的生物活性化合物和生物活性。在这项研究中，已经测试了不同的超声辅助提取（UAE）方法从CSS提取生物活性化合物。使用新的HPLC-MS / MS方法对获得的提取物进行表征，以检测和定量两种生物活性化合物，分别为生物碱和多酚（包括酚酸，类黄酮和类硒化物）两类。以乙醇/水（70:30）为萃取溶剂的CSS萃取物含量最高（p≤0.05）的生物活性化合物（4.01±0.34％w / w）。观察到咖啡因含量高，含量为提取物干重（dw）的1.00％至3.59％。用咖啡酰奎尼酸（3-CQA，5-CQA和3,5-diCQA）作为最丰富的多酚（3115.6 µg g ^-1至5444.0 µg g ^-1）在CSS提取物中检测到18种酚类化合物。这项研究还是第一个表征CSS中深度类黄酮的化合物，它揭示了不同类黄酮化合物的水平，例如芦丁（1.63 – 8.70 µg g ^-1），槲皮素（1.53 – 2.46 µg g ^-1），山emp酚（0.76 – 1.66 µg g ^-1）和槲皮素（0.15 – 0.51 µg g ^-1）。银皮提取物对H _2的神经保护活性首次评估了O ₂诱导的损伤，表明甲醇和乙醇/水（70:30）提取物具有潜在的作用，因为它们具有抗氧化应激和维持细胞活力的能力，因此可作为抗神经退行性疾病的保护剂。银皮提取物并未抑制本研究中任何细菌的生长，但水提取物获得的数据可能值得对生物膜相关活性（例如群体感应或毒力因子的表达）进行更深入的研究。从其成分和已证实的生物活性来看，CSS提取物可以代表营养制剂中的宝贵成分。