Correct selection of the cell division axis is important for cell differentiation, tissue and organ morphogenesis, and homeostasis. Both integrins, which mediate interactions with extracellular matrix (ECM) components such as fibronectin, and cadherins, which mediate interactions between cells, are implicated in the determination of spindle orientation. We found that both cadherin- and integrin-based adhesion resulted in cell divisions parallel to the attachment plane and elicited identical spindle responses to spatial adhesive cues. This suggests that adhesion topology provides purely mechanical spatial cues that are independent of the molecular nature of the interaction or signaling from adhesion complexes. We also demonstrated that cortical integrin activation was indispensable for correct spindle orientation on both cadherin and fibronectin substrates. These data suggest that spindle orientation responses to adhesion topology are primarily a result of force anisotropy on the cell cortex and show that integrins play a central role in this process that is distinct from their role in cell-ECM interactions.

中文翻译：

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有丝分裂细胞对底物拓扑线索的反应与粘附的分子性质无关。

正确选择细胞分裂轴对于细胞分化、组织和器官形态发生以及体内平衡很重要。介导与细胞外基质 (ECM) 成分（如纤连蛋白）相互作用的整联蛋白和介导细胞间相互作用的钙粘蛋白都与纺锤体方向的确定有关。我们发现基于钙粘蛋白和整合素的粘附导致平行于附着平面的细胞分裂，并引发对空间粘附线索的相同纺锤体反应。这表明粘附拓扑提供了纯粹的机械空间线索，这些线索与粘附复合物的相互作用或信号传递的分子性质无关。我们还证明了皮质整合素激活对于钙粘蛋白和纤连蛋白基质上的正确纺锤体方向是必不可少的。这些数据表明，纺锤体方向对粘附拓扑结构的反应主要是细胞皮层力各向异性的结果，并表明整合素在这一过程中发挥着核心作用，这与它们在细胞-ECM 相互作用中的作用不同。