MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs.,Advanced Science

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MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs.
Advanced Science ( IF 14.3 ) Pub Date : 2020-02-25 , DOI: 10.1002/advs.201903185
Jingtan Zhu _{1,

2} , Tingting Yu _{1,

2} , Yusha Li _{1,

2} , Jianyi Xu _{1,

2} , Yisong Qi _{1,

2} , Yingtao Yao _{1,

2} , Yilin Ma _{1,

2} , Peng Wan _{1,

2} , Zhilong Chen _{1,

2} , Xiangning Li _{1,

2} , Hui Gong _{1,

2} , Qingming Luo _{1,

2} , Dan Zhu _{1,

2}

Affiliation

Tissue optical clearing techniques have provided important tools for large-volume imaging. Aqueous-based clearing methods are known for good fluorescence preservation and scalable size maintenance, but are limited by long incubation time, insufficient clearing performance, or requirements for specialized devices. Additionally, few clearing methods are compatible with widely used lipophilic dyes while maintaining high clearing performance. Here, to address these issues, m-xylylenediamine (MXDA) is firstly introduced into tissue clearing and used to develop a rapid, highly efficient aqueous clearing method with robust lipophilic dyes compatibility, termed MXDA-based Aqueous Clearing System (MACS). MACS can render whole adult brains highly transparent within 2.5 days and is also applicable for other intact organs. Meanwhile, MACS possesses ideal compatibility with multiple probes, especially for lipophilic dyes. MACS achieves 3D imaging of the intact neural structures labeled by various techniques. Combining MACS with DiI labeling, MACS allows reconstruction of the detailed vascular structures of various organs and generates 3D pathology of glomeruli tufts in healthy and diabetic kidneys. Therefore, MACS provides a useful method for 3D mapping of intact tissues and is expected to facilitate morphological, physiological, and pathological studies of various organs.

中文翻译：

MACS：用于完整器官 3D 绘图的快速水液清除系统。

组织光学透明技术为大体积成像提供了重要的工具。水基透明方法以良好的荧光保存和可扩展的尺寸维持而闻名，但受到孵育时间长、透明性能不足或需要专用设备的限制。此外，很少有清除方法能够与广泛使用的亲脂性染料兼容，同时保持高清除性能。为了解决这些问题，首先将间苯二甲胺（MXDA）引入组织透明化中，并用于开发一种快速、高效且具有强大亲脂性染料兼容性的水性透明方法，称为基于 MXDA 的水性透明系统（MACS）。 MACS可以在2.5天内使整个成人大脑高度透明，也适用于其他完整的器官。同时，MACS与多种探针具有理想的兼容性，特别是对于亲脂性染料。 MACS 实现了通过各种技术标记的完整神经结构的 3D 成像。 MACS 与 DiI 标记相结合，可以重建各种器官的详细血管结构，并生成健康和糖尿病肾脏中肾小球簇的 3D 病理学。因此，MACS为完整组织的3D绘图提供了一种有用的方法，并有望促进各种器官的形态学、生理学和病理学研究。

更新日期：2020-04-21

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