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CBSX3-Trxo-2 regulates ROS generation of mitochondrial complex II (succinate dehydrogenase) in Arabidopsis
Plant Science ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.plantsci.2020.110458
Jin Seok Shin , Won Mi So , Soo Youn Kim , Minsoo Noh , Sujin Hyoung , Kyoung Shin Yoo , Jeong Sheop Shin

Despite being toxic at a high concentrations, reactive oxygen species (ROS) play a pivotal role as signaling molecules in responses to stress and regulation of plant development. The mitochondrial electron transport chain (ETC) is the major source of ROS in cells. Although the regulation of ROS in mitochondria has been well elucidated, the protein-protein interaction-based regulation of ETC members has not been well elucidated. In this study, we identified a CBS domain-containing protein, CBSX3, and found that CBSX3 activates o-type thioredoxin (Trx-o2) in mitochondria. In addition, we found that Trx-o2 interacts with SDH1, a subunit of ETC complex II. Knockdown (KD) of CBSX3 revealed anther indehiscence due to deficient lignin deposition caused by insufficient ROS accumulation, and increased expression of genes related to cell cycle and accelerated plant growth. However, in the CBSX3-overexpression plants, ROS accumulation increased, and cell cycle-related gene expression decreased, and thereby plant growth was retarded and leaf size decreased. Moreover, KD of CBSX3 and Trx-o2 conferred resistance to mitochondria ETC inhibitors in terms of ROS release. Taken together, we suggest that CBSX3-Trx-o2 is a ROS generation regulator of mitochondria in plants and plays an important role in regulating plant development and the redox system.

中文翻译:

CBSX3-Trxo-2 调节拟南芥中线粒体复合物 II(琥珀酸脱氢酶)的 ROS 生成

尽管在高浓度下有毒,但活性氧 (ROS) 作为信号分子在应对胁迫和调节植物发育方面发挥着关键作用。线粒体电子传递链 (ETC) 是细胞中 ROS 的主要来源。尽管线粒体中 ROS 的调节已经得到很好的阐明,但 ETC 成员的基于蛋白质-蛋白质相互作用的调节尚未得到很好的阐明。在这项研究中,我们鉴定了一种包含 CBS 结构域的蛋白质 CBSX3,并发现 CBSX3 激活线粒体中的 o 型硫氧还蛋白 (Trx-o2)。此外,我们发现 Trx-o2 与 ETC 复合物 II 的亚基 SDH1 相互作用。敲除 (KD) CBSX3 显示由于 ROS 积累不足导致木质素沉积不足导致花药不开裂,与细胞周期相关的基因表达增加,加速植物生长。然而,在CBSX3过表达植物中,ROS积累增加,细胞周期相关基因表达减少,从而导致植物生长延迟和叶片尺寸减小。此外,CBSX3 和 Trx-o2 的 KD 在 ROS 释放方面赋予对线粒体 ETC 抑制剂的抗性。总之,我们认为 CBSX3-Trx-o2 是植物线粒体的 ROS 生成调节剂,在调节植物发育和氧化还原系统中起重要作用。CBSX3 和 Trx-o2 的 KD 在 ROS 释放方面赋予对线粒体 ETC 抑制剂的抗性。总之,我们认为 CBSX3-Trx-o2 是植物线粒体的 ROS 生成调节剂,在调节植物发育和氧化还原系统中起重要作用。CBSX3 和 Trx-o2 的 KD 在 ROS 释放方面赋予对线粒体 ETC 抑制剂的抗性。总之,我们认为 CBSX3-Trx-o2 是植物线粒体的 ROS 生成调节剂,在调节植物发育和氧化还原系统中起重要作用。
更新日期:2020-05-01
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