Atypical Resistance Protein RPW8/HR Triggers Oligomerization of the NLR Immune Receptor RPP7 and Autoimmunity.,Cell Host & Microbe

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Atypical Resistance Protein RPW8/HR Triggers Oligomerization of the NLR Immune Receptor RPP7 and Autoimmunity.
Cell Host & Microbe ( IF 20.6 ) Pub Date : 2020-02-11 , DOI: 10.1016/j.chom.2020.01.012
Lei Li ₁ , Anette Habring ₁ , Kai Wang ₂ , Detlef Weigel ₁

Affiliation

In certain plant hybrids, immunity signaling is initiated when immune components interact in the absence of a pathogen trigger. In Arabidopsis thaliana, such autoimmunity and cell death are linked to variants of the NLR RPP7 and the RPW8 proteins involved in broad-spectrum resistance. We uncover the molecular basis for this autoimmunity and demonstrate that a homolog of RPW8, HR4Fei-0, can trigger the assembly of a higher-order RPP7 complex, with autoimmunity signaling as a consequence. HR4Fei-0-mediated RPP7 oligomerization occurs via the RPP7 C-terminal leucine-rich repeat (LRR) domain and ATP-binding P-loop. RPP7 forms a higher-order complex only in the presence of HR4Fei-0 and not with the standard HR4 variant, which is distinguished from HR4Fei-0 by length variation in C-terminal repeats. Additionally, HR4Fei-0 can independently form self-oligomers, which directly kill cells in an RPP7-independent manner. Our work provides evidence for a plant resistosome complex and the mechanisms by which RPW8/HR proteins trigger cell death.

中文翻译：

非典型耐药蛋白 RPW8/HR 触发 NLR 免疫受体 RPP7 寡聚化和自身免疫。

在某些植物杂交中，当免疫成分在没有病原体触发的情况下相互作用时，免疫信号就会启动。在拟南芥中，这种自身免疫和细胞死亡与涉及广谱抗性的 NLR RPP7 和 RPW8 蛋白的变体有关。我们揭示了这种自身免疫的分子基础，并证明 RPW8 的同源物 HR4Fei-0 可以触发高阶 RPP7 复合物的组装，从而产生自身免疫信号传导。 HR4Fei-0 介导的 RPP7 寡聚化通过 RPP7 C 端富含亮氨酸的重复 (LRR) 结构域和 ATP 结合 P 环发生。 RPP7 仅在 HR4Fei-0 存在的情况下形成高阶复合物，而不是与标准 HR4 变体形成高阶复合物，标准 HR4 变体与 HR4Fei-0 的区别在于 C 端重复序列的长度变化。此外，HR4Fei-0可以独立形成自身寡聚体，以不依赖RPP7的方式直接杀死细胞。我们的工作为植物抗性体复合物以及 RPW8/HR 蛋白触发细胞死亡的机制提供了证据。

更新日期：2020-02-25

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