Red blood cell membrane-coated upconversion nanoparticles for pretargeted multimodality imaging of triple-negative breast cancer,Biomaterials Science

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Red blood cell membrane-coated upconversion nanoparticles for pretargeted multimodality imaging of triple-negative breast cancer
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-02-25 , DOI: 10.1039/d0bm00029a
Mengting Li _{1,

2,

3,

4,

5} , Hanyi Fang _{1,

2,

3,

4,

5} , Qingyao Liu _{1,

2,

3,

4,

5} , Yongkang Gai _{1,

2,

3,

4,

5} , Lujie Yuan _{1,

2,

3,

4,

5} , Sheng Wang _{3,

4,

6,

7,

8} , Huiling Li _{1,

2,

3,

4,

5} , Yi Hou _{9,

10,

11,

12,

13} , Mingyuan Gao _{14,

15,

16,

17,

18} , Xiaoli Lan _{1,

2,

3,

4,

5}

Affiliation

Department of Nuclear Medicine
Union Hospital
Tongji Medical College
Huazhong University of Science and Technology
Wuhan 430022
School of Pharmacy
Wuhan 430030
China
Key Laboratory of Colloid
Interface and Chemical Thermodynamics
Institute of Chemistry
Chinese Academy of Sciences
Beijing 100190
Center for Molecular Imaging and Nuclear Medicine
State Key Laboratory of Radiation Medicine and Protection
School for Radiological and Interdisciplinary Sciences (RAD-X)
Soochow University
Suzhou 215123

Upconversion nanoparticles (UCNPs) have been widely employed for tumor imaging using magnetic resonance imaging (MRI) and upconversion luminescence (UCL) imaging. The short blood clearance time and immunogenicity of UCNPs have limited their further application in vivo. We have designed UCNPs camouflaged with an exterior red blood cell (RBC) membrane coating (RBC-UCNPs) to solve these problems. Moreover, because of some intrinsic disadvantages of MRI and UCL imaging, we investigated the use of pretargeted RBC-UCNPs for positron-emission tomography (PET) imaging to obtain more comprehensive information. Our data showed that RBC-UCNPs retained the immunity feature from the source cells and the superior optical and chemical features from the pristine UCNP cores. The tumor-targeting ability of RBC-UCNPs was enhanced by binding 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-2000] (DSPE-PEG-FA) molecules onto the cell membranes. PET imaging with short half-life radionuclides to visualize the RBC-UCNPs was successfully realized by a combination of pre-targeting and in vivo click chemistry. Blood chemistry, hematology, and histologic analysis suggested good in vivo biocompatibility of the RBC-UCNPs. Our method provides a new potential biomedical application of biomimetic nanoparticles.

中文翻译：

红细胞膜包裹的上转换纳米粒子用于三阴性乳腺癌的预靶向多峰成像

上转换纳米颗粒（UCNP）已被广泛用于使用磁共振成像（MRI）和上转换发光（UCL）成像的肿瘤成像。UCNPs的血液清除时间短和免疫原性限制了它们在体内的进一步应用。我们设计了带有外部红细胞（RBC）膜涂层（RBC-UCNPs）的UCNP，以解决这些问题。此外，由于MRI和UCL成像的某些固有缺点，我们研究了将预靶向RBC-UCNP用于正电子发射断层扫描（PET）成像以获得更全面的信息。我们的数据表明，RBC-UCNPs保留了源细胞的免疫功能以及原始UCNP核心的卓越光学和化学功能。通过结合1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N增强RBC-UCNPs的肿瘤靶向能力-[叶酸（聚乙二醇）-2000]（DSPE-PEG-FA）分子进入细胞膜。通过预靶向和体内点击化学的结合，成功实现了具有短半衰期放射性核素以可视化RBC-UCNP的PET成像。血液化学，血液学和组织学分析表明，RBC-UCNP具有良好的体内生物相容性。我们的方法提供了仿生纳米颗粒的新的潜在生物医学应用。

更新日期：2020-02-25

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