Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets through which pathogens enter target cells. These receptor roles have often been documented with binding studies involving individual pairs of receptors and microorganisms. To provide a systematic overview of interactions between microbes and the large complement of C-type lectins, here we developed a lectin array and suitable protocols for labeling of microbes that could be used to probe this array. The array contains C-type lectins from cow, chosen as a model organism of agricultural interest for which the relevant pathogen–receptor interactions have not been previously investigated in detail. Screening with yeast cells and various strains of both Gram-positive and -negative bacteria revealed distinct binding patterns, which in some cases could be explained by binding to lipopolysaccharides or capsular polysaccharides, but in other cases they suggested the presence of novel glycan targets on many of the microorganisms. These results are consistent with interactions previously ascribed to the receptors, but they also highlight binding to additional sugar targets that have not previously been recognized. Our findings indicate that mammalian lectin arrays represent unique discovery tools for identifying both novel ligands and new receptor functions.

中文翻译：

---

哺乳动物凝集素阵列用于筛选宿主与微生物的相互作用

C型凝集素聚糖结合受体家族的许多成员已被赋予在识别微生物中的作用，并在对病原体的先天免疫应答中用作关键受体。其他哺乳动物受体已成为病原体进入靶细胞的靶标。这些受体的作用通常已通过涉及单个受体对和微生物对的结合研究得到了证明。为了提供微生物与C型凝集素的大量补体之间相互作用的系统概述，在这里我们开发了一种凝集素阵列和适用于微生物标记的合适规程，可用于探测该阵列。该阵列包含来自母牛的C型凝集素，被选作具有农业意义的典范生物，其相关的病原体-受体相互作用以前没有经过详细研究。用酵母细胞和各种革兰氏阳性和阴性细菌菌株进行筛选显示出独特的结合模式，在某些情况下可以通过与脂多糖或荚膜多糖结合来解释，但在其他情况下，它们表明在许多细菌上存在新的聚糖靶标的微生物。这些结果与先前归因于受体的相互作用是一致的，但是它们还强调了与先前未认识到的其他糖靶的结合。我们的发现表明，哺乳动物凝集素阵列代表了独特的发现工具，可用于识别新型配体和新型受体功能。在某些情况下，这可以通过与脂多糖或荚膜多糖结合来解释，但在其他情况下，他们建议在许多微生物上存在新的聚糖靶标。这些结果与先前归因于受体的相互作用是一致的，但是它们还强调了与先前未认识到的其他糖靶的结合。我们的发现表明，哺乳动物凝集素阵列代表了独特的发现工具，可用于识别新型配体和新型受体功能。在某些情况下，这可以通过与脂多糖或荚膜多糖结合来解释，但在其他情况下，他们建议在许多微生物上存在新的聚糖靶标。这些结果与先前归因于受体的相互作用是一致的，但是它们还强调了与先前未认识到的其他糖靶的结合。我们的发现表明，哺乳动物凝集素阵列代表了独特的发现工具，可用于识别新型配体和新型受体功能。