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Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-01-31 , DOI: 10.1002/ppsc.201900441 Yanxia Xu 1 , Yanbing Lv 1 , Ruili Wu 1 , Huaibin Shen 1 , Huawei Yang 1 , Han Zhang 1 , Jinjie Li 1 , Lin Song Li 1
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-01-31 , DOI: 10.1002/ppsc.201900441 Yanxia Xu 1 , Yanbing Lv 1 , Ruili Wu 1 , Huaibin Shen 1 , Huawei Yang 1 , Han Zhang 1 , Jinjie Li 1 , Lin Song Li 1
Affiliation
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Indium phosphide (InP) quantum dots (QDs) are ideal substitutes for widely used cadmium‐based QDs and have great application prospects in biological fields due to their environmentally benign properties and human safety. However, the synthesis of InP core/shell QDs with biocompatibility, high quantum yield (QY), uniform particle size, and high stability is still a challenging subject. Herein, high quality (QY up to 72%) thick shell InP/GaP/ZnS core/shell QDs (12.8 ± 1.4 nm) are synthesized using multiple injections of shell precursor and extension of shell growth time, with GaP serving as the intermediate layer and 1‐octanethiol acting as the new S source. The thick shell InP/GaP/ZnS core/shell QDs still keep high QY and photostability after transfer into water. InP/GaP/ZnS core/shell QDs as fluorescence labels to establish QD‐based fluorescence‐linked immunosorbent assay (QD‐FLISA) for quantitative detection of C‐reactive protein (CRP), and a calibration curve is established between fluorescence intensity and CRP concentrations (range: 1–800 ng mL−1, correlation coefficient: R2 = 0.9992). The limit of detection is 2.9 ng mL−1, which increases twofold compared to previously reported cadmium‐free QD‐based immunoassays. Thus, InP/GaP/ZnS core/shell QDs as a great promise fluorescence labeling material, provide a new route for cadmium‐free sensitive and specific immunoassays in biomedical fields.
中文翻译：
高度稳定和光致发光的无镉InP / GaP / ZnS核/壳量子点的制备及其在定量免疫分析中的应用
磷化铟（InP）量子点（QD）是广泛使用的基于镉的QD的理想替代品，由于它们的环境友好特性和人体安全性，在生物领域具有广阔的应用前景。然而，具有生物相容性，高量子产率（QY），均匀的粒径和高稳定性的InP核/壳QD的合成仍然是具有挑战性的课题。在此，通过多次注入壳前驱体并延长壳生长时间，以GaP作为中间层，合成了高质量（QY高达72％）厚的壳InP / GaP / ZnS核/壳QD（12.8±1.4 nm）和1-辛烷硫醇作为新的S源。厚壳InP / GaP / ZnS核/壳量子点转移到水中后仍保持较高的QY和光稳定性。-1，相关系数：R 2 = 0.9992）。检测限为2.9 ng mL -1，与以前报道的基于QD的无镉免疫分析法相比，检测限增加了两倍。因此，InP / GaP / ZnS核/壳量子点作为一种很有前途的荧光标记材料，为生物医学领域的无镉敏感和特异性免疫测定提供了一条新途径。
更新日期：2020-01-31
中文翻译：
高度稳定和光致发光的无镉InP / GaP / ZnS核/壳量子点的制备及其在定量免疫分析中的应用
磷化铟（InP）量子点（QD）是广泛使用的基于镉的QD的理想替代品，由于它们的环境友好特性和人体安全性，在生物领域具有广阔的应用前景。然而，具有生物相容性，高量子产率（QY），均匀的粒径和高稳定性的InP核/壳QD的合成仍然是具有挑战性的课题。在此，通过多次注入壳前驱体并延长壳生长时间，以GaP作为中间层，合成了高质量（QY高达72％）厚的壳InP / GaP / ZnS核/壳QD（12.8±1.4 nm）和1-辛烷硫醇作为新的S源。厚壳InP / GaP / ZnS核/壳量子点转移到水中后仍保持较高的QY和光稳定性。-1，相关系数：R 2 = 0.9992）。检测限为2.9 ng mL -1，与以前报道的基于QD的无镉免疫分析法相比，检测限增加了两倍。因此，InP / GaP / ZnS核/壳量子点作为一种很有前途的荧光标记材料，为生物医学领域的无镉敏感和特异性免疫测定提供了一条新途径。
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