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Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-01-12 , DOI: 10.1002/bit.27269
Matthew N Henry 1 , Michael A MacDonald 2 , Camila A Orellana 1 , Peter P Gray 1 , Marianne Gillard 1 , Kym Baker 2, 3 , Lars K Nielsen 1, 2, 4, 5 , Esteban Marcellin 1, 2, 4 , Stephen Mahler 2 , Verónica S Martínez 2
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Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.
更新日期:2020-03-09
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