Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.,Biotechnology and Bioengineering

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Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-01-12 , DOI: 10.1002/bit.27269
Matthew N Henry ₁ , Michael A MacDonald ₂ , Camila A Orellana ₁ , Peter P Gray ₁ , Marianne Gillard ₁ , Kym Baker _{2,

3} , Lars K Nielsen _{1,

2,

4,

5} , Esteban Marcellin _{1,

2,

4} , Stephen Mahler ₂ , Verónica S Martínez ₂

Affiliation

Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.

更新日期：2020-03-09

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