Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L.,Global Change Biology Bioenergy

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Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L.
Global Change Biology Bioenergy ( IF 5.9 ) Pub Date : 2020-01-22 , DOI: 10.1111/gcbb.12667
Jacob A. Toth ₁ , George M. Stack ₁ , Ali R. Cala ₂ , Craig H. Carlson ₁ , Rebecca L. Wilk ₁ , Jamie L. Crawford ₃ , Donald R. Viands ₃ , Glenn Philippe ₄ , Christine D. Smart ₂ , Jocelyn K. C. Rose ₄ , Lawrence B. Smart ₁

Affiliation

Hemp (Cannabis sativa L.) is an emerging dioecious crop grown primarily for grain, fiber, and cannabinoids. There is good evidence for medicinal benefits of the most abundant cannabinoid in hemp, cannabidiol (CBD). For CBD production, female plants producing CBD but not tetrahydrocannabinol (THC) are desired. We developed and validated high‐throughput PACE (PCR Allele Competitive Extension) assays for C. sativa plant sex and cannabinoid chemotype. The sex assay was validated across a wide range of germplasm and resolved male plants from female and monoecious plants. The cannabinoid chemotype assay revealed segregation in hemp populations, and resolved plants producing predominantly THC, predominantly CBD, and roughly equal amounts of THC and CBD. Cultivar populations that were thought to be stabilized for CBD production were found to be segregating phenotypically and genotypically. Many plants predominantly producing CBD accumulated more than the current US legal limit of 0.3% THC by dry weight. These assays and data provide potentially useful tools for breeding and early selection of hemp.

中文翻译：

大麻的性别和大麻素化学型遗传标记的开发和验证

大麻（Cannabis sativa L.）是一种新兴的雌雄异体作物，主要种植谷物，纤维和大麻素。有充分的证据证明大麻中大麻素含量最高的大麻素（CBD）具有药用价值。对于CBD的生产，需要产生CBD而不是四氢大麻酚（THC）的雌性植物。我们开发并验证了用于苜蓿假丝酵母的高通量PACE（PCR等位基因竞争性延伸）测定方法植物性和大麻素化学型。性别分析已在广泛的种质和雌性和雌雄同株的雄性植物中得到验证。大麻素化学型分析揭示了大麻种群中的分离现象，并且分解出的植物主要产生THC，主要为CBD，并产生等量的THC和CBD。发现被认为对CBD生产稳定的品种种群在表型和基因型上隔离。许多主要生产CBD的工厂积累的水量超过了美国现行法律规定的干重0.3％THC。这些分析和数据为大麻的育种和早期选择提供了潜在有用的工具。

更新日期：2020-01-22

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