The synthesis of magnetic Hematite nanoparticles (α-Fe₂O₃) via green route has been a long lasting challenge for the scientific and technological fascination of many researchers. In the present investigation, iron oxide nanoparticles (α-Fe₂O₃) were synthesized using Rheum emodi roots in a cost effective and ecofriendly method. Their physiochemical property orchestration involved techniques such as UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray (EDX), X-ray diffraction (XRD), Thermogravimetric Analysis (TGA), Vibrating sample magnetometer (VSM), and Atomic force microscopy (AFM). Through TEM, FESEM and AFM analysis, α-Fe₂O₃ NPs were confirmed spherical in shape and the average diameter of particle is ~12 nm as depicted through TEM image. Thermal property was investigated by TGA. Magnetic behavior was observed in R. emodi mediated α-Fe₂O₃NPs by magnetic hysteresis measurements. FTIR analysis revealed the presence of anthraquinone in R. emodi roots extract which play the central role in stabilization of the α-Fe₂O₃NPs. Further, the crystalline nature of the nanoparticle sample was determined with XRD experiment and SAED fringes calculation. The crystal was also confirmed with Rietveld refinement of XRD profile fitted with R-3c model Additionally, magnetic interaction with bacterial cell wall showed antimicrobial property against Escherichia coli, Gram-negative and Staphylococcus aureus, Gram-positive species. The approach transcribed in this paper reveals a novel methodology that utilizes α-Fe₂O₃ NPs to initiate apoptosis and inhibition of cervical cancer cells.

通过绿色路线合成磁性赤铁矿纳米颗粒（α-Fe ₂ O ₃ ）一直是许多研究人员所着迷的科学和技术挑战。在本研究中，利用大黄根以经济有效且环保的方法合成氧化铁纳米粒子（α-Fe ₂ O ₃ ）。它们的理化性质协调涉及紫外可见光谱、傅里叶变换红外光谱 (FTIR)、透射电子显微镜 (TEM)、场发射扫描电子显微镜 (FESEM)、能量色散 X 射线 (EDX)、X 射线等技术衍射 (XRD)、热重分析 (TGA)、振动样品磁力计 (VSM) 和原子力显微镜 (AFM)。通过TEM、FESEM和AFM分析，证实α-Fe ₂ O ₃ NPs呈球形，如T​​EM图像所示，颗粒的平均直径约为12 nm。通过TGA研究热性能。在R中观察到磁性行为。 emodi通过磁滞测量介导 α-Fe ₂ O ₃ NP。 FTIR 分析表明R中存在蒽醌。 Emodi根提取物在稳定 α-Fe ₂ O ₃ NP 方面发挥着核心作用。此外，通过 XRD 实验和 SAED 条纹计算确定了纳米颗粒样品的结晶性质。 该晶体还通过与R-3c模型相匹配的 XRD 轮廓的 Rietveld 精修得到证实。此外，与细菌细胞壁的磁性相互作用显示出对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抗菌特性。本文转录的方法揭示了一种利用 α-Fe ₂ O ₃ NP 启动细胞凋亡和抑制宫颈癌细胞的新方法。