Without perfusable vascular networks, 3-D tissues populated with cells cannot maintain a living condition. To construct 3-D tissues, a well-organized vascular network is required. In this article, a method for constructing a hepatic-lobule-like vascular network in a 3-D cellular structure by using magnetic fields is employed. To realize channel networks that mimic the hepatic lobule, steel rods and magnetic fibers were utilized as a sacrificial mold in fibrin gel. The steel rods and fibers were connected by magnetic fields using magnetic tweezers. In our previous work “Construction of hepatic lobule-like vascular network by using magnetic fields” (Int. Conf. Robot. Autom., 2018, pp. 2688-2693), the tweezers were designed by 2-D simulation data. In addition, a 3-D cellular structure without a channel network was only cultured. New tweezers were designed based on 3-D simulation data to generate higher magnetic fields than the former tweezer. In addition, a tissue with the 3-D channel network was cultured for a week. To verify that the channel network can supply the nutrients to the cells in tissues, the viability of the cells located on the structure was analyzed. The cells close to the channel network show a higher cell viability than the cells far from the channel network.

中文翻译：

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操纵纤维在细胞结构中构建肝小叶样3-D血管网络

如果没有可灌注的血管网络，则充满细胞的3-D组织将无法维持生存状态。为了构建3-D组织，需要组织良好的血管网络。在本文中，采用了一种通过使用磁场在3-D细胞结构中构建肝小叶状血管网络的方法。为了实现模仿肝小叶的通道网络，钢条和磁性纤维被用作纤维蛋白凝胶中的牺牲霉菌。使用磁镊子通过磁场将钢棒和纤维连接起来。在我们之前的工作“通过使用磁场构建肝小叶样血管网络”（国际机器人学会会刊，2018年，第2688-2693页）中，镊子是通过二维模拟数据设计的。另外，仅培养了没有通道网络的3-D细胞结构。根据3-D模拟数据设计了新型镊子，以产生比以前的镊子更高的磁场。另外，将具有3-D通道网络的组织培养一周。为了验证通道网络可以为组织中的细胞提供营养，分析了位于结构上的细胞的生存能力。靠近通道网络的细胞显示出比远离通道网络的细胞更高的细胞活力。