Recently, several non-classical functions of histone modification regulators (HMRs), independent of their known histone modification substrates and products, have been reported to be essential for specific cellular processes. However, there is no framework designed for identifying such functions systematically. Here, we develop ncHMR detector, the first computational framework to predict non-classical functions and cofactors of a given HMR, based on ChIP-seq data mining. We apply ncHMR detector in ChIP-seq data-rich cell types and predict non-classical functions of HMRs. Finally, we experimentally reveal that the predicted non-classical function of CBX7 is biologically significant for the maintenance of pluripotency.

中文翻译：

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ncHMR 检测器：系统揭示组蛋白修饰调节因子非经典功能的计算框架


最近，据报道，组蛋白修饰调节剂（HMR）的几种非经典功能，与其已知的组蛋白修饰底物和产物无关，对特定的细胞过程至关重要。然而，没有设计用于系统地识别这些功能的框架。在这里，我们开发了 ncHMR 检测器，这是第一个基于 ChIP-seq 数据挖掘来预测给定 HMR 的非经典函数和辅因子的计算框架。我们将 ncHMR 检测器应用于 ChIP-seq 数据丰富的细胞类型，并预测 HMR 的非经典功能。最后，我们通过实验揭示了 CBX7 的预测非经典功能对于维持多能性具有生物学意义。