Vibrio parahaemolyticus is a common foodborne pathogen in seafood and represents a major threat to human health worldwide. In this study, we identified that PhoR, a histidine kinase, is involved in the regulation of swarming and flagella assembly. RNA sequencing analysis showed that 1122 genes were differentially expressed in PhoR mutant, including 394 upregulated and 728 downregulated genes. KEGG enrichment and heatmap analysis demonstrated that the bacterial secretion system, flagella assembly and chemotaxis pathways were significantly downregulated in PhoR mutant, while the microbial metabolism in diverse environments and carbon metabolism pathways were upregulated in PhoR mutant. qRT-PCR further confirmed that genes responsible for the type III secretion system (T3SS), swarming and the thermostable direct hemolysin were positively regulated by PhoR. Phosphorylation assays suggested that PhoR was highly activated in BHI medium compared to LB medium. Taken together, these data suggested that activated PhoR contributes to the expression of swarming motility and secretion system genes in Vibrio parahaemolyticus.

副溶血性弧菌是海鲜中常见的食源性病原体，对全球人类健康构成重大威胁。在这项研究中，我们确定了PhoR，一种组氨酸激酶，参与了群聚和鞭毛组装的调控。RNA测序分析表明，在PhoR突变体中1122个基因差异表达，包括394个上调基因和728个下调基因。KEGG富集和热图分析表明，PhoR突变体中的细菌分泌系统，鞭毛组装和趋化途径显着下调，而不同环境中的微生物代谢和碳代谢途径则被PhoR突变体上调。qRT-PCR进一步证实，负责PhoR的基因正调控着III型分泌系统（T3SS），蜂群和热稳定的直接溶血素。磷酸化分析表明，与LB培养基相比，Bhi培养基中的PhoR被高度激活。综上所述，这些数据表明，活化的PhoR促进了成群运动和分泌系统基因的表达。副溶血性弧菌。