Streptomyces and their biomolecules are well explored for antibiotics production, bioremediation and alleviating the plant stresses due to their plant beneficial attributes. Therefore, due to plethora of biological attributes, the accurate portraying of molecular capabilities of these microorganisms at genomic level is of paramount importance. Here, we have evaluated biochemical attributes of two Streptomyces sp. AC30and AC40 for different plant beneficial activities which are antagonistic to Fusarium oxysporum, Alternaria solani, Sclerotinia sclerotium and Phytopthora infestans. In parallel, the draft genomes of these strains were deduced to understand their genomic capabilities using Illumina platform. The complete genome of AC30and AC40 were 11,284,599 bp and 12,636,188 bp in size with total G + C content of 62.36 and 54.75 %, respectively. Overall, higher number of genes (14,024) was reported for AC40 as compared to AC30 (12,476). The comparative genome organization revealed sharing of a few biosynthetic clusters as well as some exclusive biosynthetic clusters among both the strains. Further, expansion in the chitinases and glucanases was found in the genome of AC40. In addition, genes for 3-phytase and glycosyl hydrolase family 19 were restricted to AC40 only. The comparative genome study revealed presence of plant induced nitrilase in AC40 which is predicted for its role in IAA biosynthesis, release of ammonia, biotransformation of nitrile compounds to corresponding acids and bioremediation of soil containing nitrile compounds. For IAA and secondary metabolites biosynthesis, flavin-dependent monooxygenase, a rate limiting factor in Trp-dependent auxin biosynthesis pathway was found exclusive to AC30 genome. The comparative study revealed the diversification of few pathways/strategies to suppress plant pathogens and promote plant growth by Streptomyces strains.

链霉菌及其生物分子因其对植物有益的特性而被广泛用于抗生素生产，生物修复和缓解植物胁迫方面。因此，由于过多的生物学特性，在基因组水平上准确描绘这些微生物的分子能力至关重要。在这里，我们评估了两个链霉菌的生化属性。AC30和AC40具有不同的植物有益活性，可对抗尖孢镰刀菌，链格孢菌，核盘菌和疫霉菌的拮抗作用。同时，使用Illumina平台推导了这些菌株的基因组草图以了解其基因组能力。AC30和AC40的完整基因组大小分别为11,284,599 bp和12,636,188 bp，总G + C含量分别为62.36和54.75％。总体而言，与AC30（12,476）相比，AC40报道的基因数量更多（14024）。比较基因组的组织揭示了两个菌株之间共享了一些生物合成簇以及一些专有的生物合成簇。此外，在AC40的基因组中发现了几丁质酶和葡聚糖酶的扩增。此外，3-植酸酶和糖基水解酶家族19的基因仅限于AC40。比较基因组研究表明，AC40中存在植物诱导的腈水解酶，据推测其在IAA生物合成，氨释放，腈化合物向相应酸的生物转化以及含腈化合物的土壤的生物修复。对于IAA和次级代谢产物的生物合成，发现黄素依赖性单加氧酶是Trp依赖性生长素生物合成途径中的限速因子，仅AC30基因组存在。比较研究表明，通过抑制植物病原体和促进植物生长的几种途径/策略的多样化链霉菌菌株。