IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.

中文翻译：

---

派生自帕金森氏病患者的诱导多能干细胞系RCPCMi004-A，其PARK2基因中的外显子2缺失。

IPSC系RCPCMi004-A是由从患有帕金森氏病的男性患者中收集的皮肤成纤维细胞产生的。该患者携带PARK2基因外显子2的杂合缺失。成纤维细胞的重编程是用含有Oct-4，Sox-2，Klf-4和c-Myc的仙台病毒进行的。通过免疫荧光，RT-PCR和类胚体的形成证实了多能性。RCPCMi004-A细胞系在PARK2基因中带有相同的缺失。RCPCMi004-A细胞系可用于体外建模帕金森氏病。