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Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-02-23 , DOI: 10.1002/adhm.201901658 Melanie Krüger 1, 2 , Loes A Oosterhoff 1 , Monique E van Wolferen 1 , Simon A Schiele 3 , Andreas Walther 4 , Niels Geijsen 1, 5 , Laura De Laporte 3 , Luc J W van der Laan 6 , Linda M Kock 2 , Bart Spee 1
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-02-23 , DOI: 10.1002/adhm.201901658 Melanie Krüger 1, 2 , Loes A Oosterhoff 1 , Monique E van Wolferen 1 , Simon A Schiele 3 , Andreas Walther 4 , Niels Geijsen 1, 5 , Laura De Laporte 3 , Luc J W van der Laan 6 , Linda M Kock 2 , Bart Spee 1
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To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting. Recently, adult liver-derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate. The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable. Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids. The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG. Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.
中文翻译：
纤维素纳米原纤维水凝胶促进人类肝脏类器官的肝分化。
为了在体外复制功能性肝组织以进行药物测试或移植，3D组织工程需要代表性的细胞模型以及支架，这些支架不仅可以促进组织生产，而且可以应用于临床。近来，由于其遗传稳定性，扩增潜力和向肝细胞样命运分化的能力，发现成年肝来源的肝类器官很受关注。用于培养这些类器官的当前标准是基底膜水凝胶，如Matrigel（MG），它源自鼠类肿瘤材料，并且由于其可变性和高成本，其组成不确定，因此不适用于临床。这里，研究了纤维素纳米原纤维（CNF）水凝胶作为替代临床级支架以区分肝类器官的潜力。结果表明，与MG相比，它的机械性能适合于肝细胞样细胞的总体功能（相等或改善）的分化。因此，由于其定义的和可调的化学定义，CNF水凝胶为肝组织工程提供了MG的可行替代品，并具有临床用途。
更新日期：2020-03-19
中文翻译：
纤维素纳米原纤维水凝胶促进人类肝脏类器官的肝分化。
为了在体外复制功能性肝组织以进行药物测试或移植，3D组织工程需要代表性的细胞模型以及支架，这些支架不仅可以促进组织生产，而且可以应用于临床。近来，由于其遗传稳定性，扩增潜力和向肝细胞样命运分化的能力，发现成年肝来源的肝类器官很受关注。用于培养这些类器官的当前标准是基底膜水凝胶，如Matrigel（MG），它源自鼠类肿瘤材料，并且由于其可变性和高成本，其组成不确定，因此不适用于临床。这里，研究了纤维素纳米原纤维（CNF）水凝胶作为替代临床级支架以区分肝类器官的潜力。结果表明，与MG相比，它的机械性能适合于肝细胞样细胞的总体功能（相等或改善）的分化。因此，由于其定义的和可调的化学定义，CNF水凝胶为肝组织工程提供了MG的可行替代品，并具有临床用途。
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