A growing body of literature indicates that activation of cannabinoid receptors may exert beneficial effects on gastrointestinal inflammation and visceral hypersensitivity. The present study aimed to immunohistochemically investigate the distribution of the canonical cannabinoid receptors CB1 (CB1R) and CB2 (CB2R) and the putative cannabinoid receptors G protein-coupled receptor 55 (GPR55), nuclear peroxisome proliferator-activated receptor alpha (PPARα), transient receptor potential ankyrin 1 (TRPA1), and serotonin receptor 5-HT1a 5-HT1aR) in tissue samples of the gastrointestinal tract of the cat. CB1R-immunoreactivity (CB1R-IR) was observed in gastric epithelial cells, intestinal enteroendocrine cells (EECs) and goblet cells, lamina propria mast cells (MCs), and enteric neurons. CB2R-IR was expressed by EECs, enterocytes, and macrophages. GPR55-IR was expressed by EECs, macrophages, immunocytes, and MP neurons. PPARα-IR was expressed by immunocytes, smooth muscle cells, and enteroglial cells. TRPA1-IR was expressed by enteric neurons and intestinal goblet cells. 5-HT1a receptor-IR was expressed by gastrointestinal epithelial cells and gastric smooth muscle cells. Cannabinoid receptors showed a wide distribution in the feline gastrointestinal tract layers. Although not yet confirmed/supported by functional evidences, the present research might represent an anatomical substrate potentially useful to support, in feline species, the therapeutic use of cannabinoids during gastrointestinal inflammatory diseases.

中文翻译：

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大麻素和大麻素相关受体在猫胃肠道中的定位。

越来越多的文献表明，大麻素受体的激活可能对胃肠道炎症和内脏超敏反应产生有益作用。本研究旨在通过免疫组织化学研究规范性大麻素受体CB1（CB1R）和CB2（CB2R）和假定的大麻素受体G蛋白偶联受体55（GPR55），核过氧化物酶体增殖物激活受体α（PPARα）的分布猫胃肠道组织样本中的受体电位锚蛋白1（TRPA1）和血清素受体5-HT1a 5-HT1aR）。在胃上皮细胞，肠肠内分泌细胞（EEC）和杯状细胞，固有层肥大细胞（MC）和肠神经元中观察到CB1R免疫反应性（CB1R-1R）。CB2R-1R由EEC，肠上皮细胞和巨噬细胞表达。GPR55-IR由EEC，巨噬细胞，免疫细胞和MP神经元表达。PPARα-IR由免疫细胞，平滑肌细胞和肠神经胶质细胞表达。肠神经元和肠杯状细胞表达TRPA1-IR。胃肠道上皮细胞和胃平滑肌细胞表达5-HT1a受体-IR。大麻素受体在猫的胃肠道层中分布较广。尽管尚未得到功能性证据的证实/支持，但本研究可能代表一种解剖底物，可能在猫科动物物种中支持胃肠炎性疾病中大麻素的治疗用途。肠神经元和肠杯状细胞表达TRPA1-IR。胃肠道上皮细胞和胃平滑肌细胞表达5-HT1a受体-IR。大麻素受体在猫的胃肠道层中分布较广。尽管尚未得到功能性证据的证实/支持，但本研究可能代表一种解剖底物，可能在猫科动物物种中支持胃肠炎性疾病中大麻素的治疗用途。肠神经元和肠杯状细胞表达TRPA1-IR。胃肠道上皮细胞和胃平滑肌细胞表达5-HT1a受体-IR。大麻素受体在猫的胃肠道层中分布较广。尽管尚未得到功能性证据的证实/支持，但本研究可能代表一种解剖底物，可能在猫科动物物种中支持胃肠炎性疾病中大麻素的治疗用途。