Hypochlorous acid (HClO)/hypochlorite (ClO⁻) play crucial roles in many physiological and pathological processes. Herein, two near-infrared fluorescent probe FDOCl-1 and FDOCl-2 were designed and synthesized for selective and sensitive detecting ClO⁻. The probe showed fast response (FDOCl-1: less than 50 s, FDOCl-2: less than 30 s), remarkable colorimetric change, excellent selectivity and sensitivity, low limit of detection (FDOCl-1: 53.5 nM, FDOCl-2: 31.7 nM) and good pH stability. Moreover, the probe FDOCl-2 could penetrate cell membranes, and was used to detect exogenous and endogenous ClO⁻ in living HeLa cells and Caenorhabditis elegans. Thus, we suggest that our newly generated probe FDOCl-2 might have potential application in the rapid and sensitive detection of ClO⁻ in vivo.

次氯酸（次氯酸）/次氯酸盐（CLO ^- ）发挥许多生理和病理过程中的关键角色。在本文中，两个近红外荧光探针FDOCl-1和FDOCl-2被设计和用于选择性和灵敏的检测CLO合成^- 。探针显示快速响应（FDOC1-1：小于50 s，FDOC1-2：小于30 s），显着的比色变化，出色的选择性和灵敏度，低检测限（FDOC1-1：53.5 nM，FDOC1-2： 31.7 nM）和良好的pH稳定性。此外，探针FDOC1-2可穿透细胞膜，并用于检测外源性和内源性ClO。⁻在活的HeLa细胞和秀丽隐杆线虫中。因此，我们认为我们的新生成的探测FDOCl-2可能在快速，灵敏的检测CLO的潜在应用^- 在体内。