In this present study, in vitro characterization on bioactivity and biocompatibility of the copper‐doped hydroxyapatite (Cu‐HA) coatings deposited onto Ti6Al4V alloy with increasing copper content obtained using solution precursor plasma spray process were evaluated under simulated body fluid (SBF) and cytotoxicity tests. The growth of flake‐like structures was observed at the coatings' surface after 7 days of immersion in SBF. Elemental composition analysis showed a calcium‐deficient carbonate‐containing apatite for the grown layer. Broadening of the HA peaks were also observed in the X‐ray diffraction patterns and infrared absorption spectra of the immersed coatings associated with the possible formation of an amorphous layer at the surface of the coatings. Copper incorporation does not alter the bioactivity of HA but only slowed down for 10 mol% copper content. Cytotoxicity evaluation using MTT assays showed that 3 mol% Cu‐HA coating was not toxic under in vitro characterizations.

中文翻译：

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随着铜含量的增加，溶液前体等离子体喷涂铜掺杂羟基磷灰石涂层的体外研究。

在本研究中，在模拟体液 (SBF) 和细胞毒性下评估了使用溶液前驱体等离子喷涂工艺获得的铜含量增加的 Ti6Al4V 合金上沉积的铜掺杂羟基磷灰石 (Cu-HA) 涂层的生物活性和生物相容性的体外表征测试。在 SBF 中浸泡 7 天后，在涂层表面观察到片状结构的生长。元素组成分析显示生长层含有缺钙的碳酸盐磷灰石。在浸渍涂层的 X 射线衍射图和红外吸收光谱中也观察到 HA 峰的增宽，这与涂层表面可能形成的非晶层有关。铜的掺入不会改变 HA 的生物活性，但只会在 10 mol% 的铜含量下减慢。使用 MTT 测定的细胞毒性评估表明，在体外表征下，3 mol% 的 Cu-HA 涂层没有毒性。