The prosthetic mesh, which is widely used in tension‐free hernioplasty, often result in avascular stiff fibrotic scar or mesh shrinkage, causing chronic pain and infection. Here, we developed an autologous bionic tissue (ABT), which was composed of autologous bone marrow‐derived mesenchymal stem cells (MSCs), poly (lactic‐co‐glycolic acid) (PLGA) porous scaffolds, and extracellular matrix (ECM) produced by MSCs for inguinal hernioplasty. In ABT, MSCs produced a variety of ECM composites, such as structural proteins (insoluble collagen, elastin) that provided mechanical properties, macromolecules (hyaluronic acid, glycosaminoglycan) as water and cytokines reservoir, and cell‐engaging proteins (fibronectin, laminin). The above ECM composites reached the highest level in 21 days. ECM degradation related cytokines (MMP‐9 and its inhibitor TIMP‐1) reached the highest level on the 14th day. ECM increased the mechanical properties, elasticity, and flexibility of PLGA. Compared with the PLGA, ABT greatly inhibited inflammatory factors and promoted anti‐inflammatory factors (p < 0.05), and gradually reduced the M1/M2 ratio in vivo (p < 0.05). After implantation, the thickness of tissue regeneration (p < 0.05), the number of capillaries or mature vessels (p < 0.05), the mechanical properties of ABT (p < 0.05) were greater than PLGA. MSCs and ECM could reduce the inflammation caused by PLGA, and prevent PLGA from earlier degradation and facilitate host cellular infiltration, thus ABT could greatly promote tissue regeneration in hernia repairs.

中文翻译：

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自体仿生组织用于腹股沟疝修复。

假体补片广泛用于无张力疝修补术，常导致无血管僵硬纤维化瘢痕或补片收缩，引起慢性疼痛和感染。在这里，我们开发了一种自体仿生组织（ABT），它由自体骨髓间充质干细胞（MSCs）、聚（乳酸-乙醇酸共聚物）（PLGA）多孔支架和产生的细胞外基质（ECM）组成。 MSCs 用于腹股沟疝修补术。在 ABT 中，MSC 产生多种 ECM 复合物，例如提供机械性能的结构蛋白（不溶性胶原蛋白、弹性蛋白）、作为水和细胞因子储库的大分子（透明质酸、糖胺聚糖）以及细胞参与蛋白（纤连蛋白、层粘连蛋白）。上述ECM复合材料达到了21天的最高水平。ECM降解相关细胞因子（MMP-9及其抑制剂TIMP-1）在第14天达到最高水平。ECM 增加了 PLGA 的机械性能、弹性和柔韧性。与PLGA相比，ABT大大抑制炎症因子并促进抗炎因子。p < 0.05)，并逐渐降低体内 M1/M2 比值 ( p < 0.05)。植入后组织再生厚度（p <0.05）、毛细血管或成熟血管数量（p <0.05）、ABT的力学性能（p <0.05）均大于PLGA。MSCs和ECM可以减轻PLGA引起的炎症，防止PLGA过早降解并促进宿主细胞浸润，因此ABT可以极大地促进疝气修复中的组织再生。